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Literature summary for 2.4.1.1 extracted from
- Glycogen-bound phosphorylase in Cryptococcus laurentii (1970), Biochim. Biophys. Acta, 215, 39-51.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not activated by AMP | Papiliotrema laurentii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Papiliotrema laurentii |  |
| ADPglucose | reversible by Mg2+ | Papiliotrema laurentii | |
| AMP | reversible by Mg2+; weak | Papiliotrema laurentii | |
| Cu2+ | - |
Papiliotrema laurentii | |
| glucose 6-phosphate | - |
Papiliotrema laurentii | |
| Mercuriacetate | - |
Papiliotrema laurentii | |
| additional information | not inhibited by 1,10-phenanthroline | Papiliotrema laurentii | |
| p-hydroxymercuribenzoate | 2 mM, 96% inhibition | Papiliotrema laurentii | |
| UDPglucose | reversible by Mg2+ | Papiliotrema laurentii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.65 mg/ml, glycogen | Papiliotrema laurentii | |
| 0.4 | - |
glucose 1-phosphate | in the presence of Mg2+ | Papiliotrema laurentii | |
| 1 | - |
glucose 1-phosphate | - |
Papiliotrema laurentii | |
| 1.5 | - |
phosphate | - |
Papiliotrema laurentii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | loosely bound to native granules of its substrate polyglucan | Papiliotrema laurentii | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Papiliotrema laurentii | |
| Co2+ | activation | Papiliotrema laurentii | |
| Mg2+ | activation | Papiliotrema laurentii | |
| Mn2+ | activation | Papiliotrema laurentii | |
| additional information | not activated by Na+ | Papiliotrema laurentii | |
| Ni2+ | activation | Papiliotrema laurentii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papiliotrema laurentii | - |
var. flavescens | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Papiliotrema laurentii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (dextrin)n-1 + glucose 1-phosphate | - |
Papiliotrema laurentii | (dextrin)n + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | glycogen from oyster | Papiliotrema laurentii | glycogen + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | endogenous glycogen | Papiliotrema laurentii | glycogen + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | glycogen from: rabbit liver | Papiliotrema laurentii | glycogen + phosphate | - |
r | |
| starch + glucose 1-phosphate | poor substrate | Papiliotrema laurentii | starch + phosphate | - |
r | |
| starch + glucose 1-phosphate | soluble starch | Papiliotrema laurentii | starch + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Papiliotrema laurentii | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Papiliotrema laurentii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 7.2 | approx. half-maximal activity at pH 5.2 and 7.2 | Papiliotrema laurentii |
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Release 2023.1 (January 2023)
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