Any feedback?
Literature summary for 2.4.1.1 extracted from
- 1,4-alpha-Glucan phosphorylase from Klebsiella pneumoniae purification, subunit structure and amino acid composition (1976), Eur. J. Biochem., 70, 291-303.
General Stability
| General Stability | Organism |
|---|---|
| phosphate protects against thermal inactivation | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by AMP | Klebsiella pneumoniae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
2 * 88000, SDS-PAGE | Klebsiella pneumoniae |
| 90000 | - |
2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl | Klebsiella pneumoniae |
| 174000 | - |
high speed sedimentation equilibrium | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, polyethylenglycol, DEAE-cellulose, CM-Sephadex | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.7 | - |
maltoheptaose | Klebsiella pneumoniae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 3.5 M (NH4)2SO4 in solution of neutral pH-value, several months, no loss of activity | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (maltodextrin)n-1 + glucose 1-phosphate | glucosylation at 96% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | (maltodextrin)n + phosphate | - |
r |  |
| amylopectin + glucose 1-phosphate | glucosylation at 23% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | amylopectin + phosphate | - |
r | |
| amylopectin + glucose 1-phosphate | waxy maize amylopectin | Klebsiella pneumoniae | amylopectin + phosphate | - |
r | |
| amylose + glucose 1-phosphate | glucosylation at 7% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | amylose + phosphate | - |
? | |
| glycogen + glucose 1-phosphate | glycogen from: rabbit liver | Klebsiella pneumoniae | glycogen + phosphate | - |
r | |
| glycogen + glucose 1-phosphate | glucosylation at 14.4% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | glycogen + phosphate | - |
r | |
| maltoheptaose + glucose 1-phosphate | - |
Klebsiella pneumoniae | maltooctaose + phosphate | - |
r | |
| maltotetraose + glucose 1-phosphate | glucosylation at 22% the rate of maltoheptaose glucosylation | Klebsiella pneumoniae | maltopentaose + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | no activity with pullulan | Klebsiella pneumoniae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | no activity with dextran | Klebsiella pneumoniae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 88000, SDS-PAGE | Klebsiella pneumoniae |
| dimer | 2 * 90000, low speed sedimentation equilibrium, after treatment with 6 M guanidine-HCl | Klebsiella pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
phosphate protects against thermal inactivation | Klebsiella pneumoniae |
| 30 | - |
30 min stable at pH 5.9-10.5 | Klebsiella pneumoniae |
| 43 | - |
10 min stable | Klebsiella pneumoniae |
| 55 | - |
loss of approx. 90% activity after 10 min in 500 mM potassium phosphate pH 7.3 | Klebsiella pneumoniae |
| 63 | - |
inactivation within 10 min | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.3 | Klebsiella pneumoniae |
| 5.9 | - |
- |
Klebsiella pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | 7.5 | approx. half-maximal activity at pH 5.1 and 7.5 | Klebsiella pneumoniae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | 10.5 | 30 min stable at 30°C | Klebsiella pneumoniae |
| 11.5 | - |
and above, denaturation | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 2 mol per mol enzyme | Klebsiella pneumoniae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
