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Literature summary for 2.3.3.9 extracted from

  • Chell, R.M.; Sundaram, T.K.
    Structural basis of the thermostability of monomeric malate synthase from a thermophilic Bacillus (1978), J. Bacteriol., 135, 334-341.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.9

Inhibitors

Inhibitors Comment Organism Structure
pyruvate
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Escherichia coli
56000
-
 gel filtration Bacillus licheniformis
56000
-
 gel filtration Vogesella indigofera
56000
-
 1 * 56000, SDS-PAGE Escherichia coli
58000
-
 1 * 58000, SDS-PAGE Bacillus sp. (in: Bacteria)
62000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus sp. (in: Bacteria)
-
 thermophilic
-
Escherichia coli
-
-
-
Vogesella indigofera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.9
-
 wild-type enzyme Bacillus sp. (in: Bacteria)
27
-
 mutant enzyme PC2 NG35 Bacillus sp. (in: Bacteria)
34.3
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + acetyl-CoA + H2O
-
 Escherichia coli (S)-malate + CoA
-
 ?
glyoxylate + acetyl-CoA + H2O
-
 Bacillus sp. (in: Bacteria) (S)-malate + CoA
-
 ?
glyoxylate + acetyl-CoA + H2O
-
 Bacillus licheniformis (S)-malate + CoA
-
 ?
glyoxylate + acetyl-CoA + H2O
-
 Vogesella indigofera (S)-malate + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE Bacillus sp. (in: Bacteria)
monomer 1 * 56000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 50 mM Tris-HCl, pH 8.0, without KCl or with 0.2 M KCl, half-life: 25 min Escherichia coli
45
-
 pH 7.5-9.5, completely stable for at least 2 h Bacillus sp. (in: Bacteria)
50
-
 50 mM Tris-HCL, pH 8.0, without KCl or with 0.2 M KCl, half-life: 3 min Escherichia coli
60
-
 25 mM Tris-HCl, pH 7.0, without KCl, half-life: 168 min, 25 mM Tris-HCL, pH 7.0, with KCl, half-life: 30 min, 25 mM glycine-NaOH buffer, pH 8.5, without KCl, half-life: 750 min, 25 mM glycine-NaOH buffer, pH 8.5, with 0.2 KCl, half-life: 50 min, 25 mM glycine-NaOH buffer, pH 9.0, without KCl, half-life: 522 min, 25 mM glycine-NaOH buffer, pH 9.0, with 0.2 KCl, half-life: 12 min, 25 mM Tris-HCL, pH 6.86, with 0.2 M KCl, half-life: 2 min Bacillus sp. (in: Bacteria)