Any feedback?
Literature summary for 2.3.3.9 extracted from
- Monomeric malate synthase from a thermophilic Bacillus (1980), Arch. Biochem. Biophys., 199, 515-525.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycolate | - |
Bacillus sp. (in: Bacteria) |  |
| oxalate | - |
Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
acetyl-CoA | - |
Bacillus sp. (in: Bacteria) | |
| 0.058 | - |
glyoxylate | - |
Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
1 * 58000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 62000 | - |
gel filtration | Neurospora crassa |
| 62000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
| 160000 | - |
gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | Escherichia coli | enzyme specifically involved in glyoxalate cycle metabolism | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | Bacillus sp. (in: Bacteria) | enzyme specifically involved in glyoxalate cycle metabolism | (S)-malate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
thermophilic | - |
| Escherichia coli | - |
- |
- |
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Bacillus sp. (in: Bacteria) | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | - |
Escherichia coli | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | - |
Neurospora crassa | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | - |
Bacillus sp. (in: Bacteria) | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | enzyme specifically involved in glyoxalate cycle metabolism | Escherichia coli | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | enzyme specifically involved in glyoxalate cycle metabolism | Bacillus sp. (in: Bacteria) | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Escherichia coli |
| monomer | - |
Neurospora crassa |
| monomer | 1 * 58000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| More | enzyme monomeric in procaryotes but multimeric in eucaryotes | Escherichia coli |
| More | enzyme monomeric in procaryotes but multimeric in eucaryotes | Neurospora crassa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27.8 | - |
acetyl-CoA | - |
Bacillus sp. (in: Bacteria) | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
4.6 | Bacillus sp. (in: Bacteria) |
| 8.6 | - |
- |
Bacillus sp. (in: Bacteria) |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
glycolate | - |
Bacillus sp. (in: Bacteria) | |
| 0.09 | - |
oxalate | - |
Bacillus sp. (in: Bacteria) | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
