Cloned (Comment) | Organism |
---|---|
into the vector pET-151 for expression in Escherichia coli BL21DE3 Star cells | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
E365Term | mutant, truncated form of NmeIPMS, loss of the regulatory domain results in a loss of the ability to catalyse the adol reaction, although the enzyme is still able to slowly hydrolyse AcCoA | Neisseria meningitidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-alpha-hydroxyisovalerate | - |
Neisseria meningitidis | |
3,3-dibromopyruvate | - |
Neisseria meningitidis | |
3-Bromopyruvate | - |
Neisseria meningitidis | |
leucine | - |
Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
3-methyl-2-oxopentanoate | pH 7.5, 25°C | Neisseria meningitidis | |
0.3 | - |
3-Fluoropyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
0.63 | - |
pyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
3.3 | - |
alpha-Ketobutyrate | pH 7.5, 25°C | Neisseria meningitidis | |
5.4 | - |
alpha-ketoisovalerate | pH 7.5, 25°C | Neisseria meningitidis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Neisseria meningitidis | |
Zn2+ | - |
Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39930 | - |
E365Term mutant, theoretical | Neisseria meningitidis |
40000 | - |
E365Term mutant, determined by SDS-PAGE | Neisseria meningitidis |
56000 | - |
wild-type enzyme, determined by SDS-PAGE | Neisseria meningitidis |
56030 | - |
wild-type enzyme, theoretical | Neisseria meningitidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Neisseria meningitidis | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | Q9JZG1 | - |
- |
Purification (Comment) | Organism |
---|---|
using Talon Superflow Metal Affinity resin, the His-tag is removed by TEV protease, anion exchange chromatography on a SourceQ column is applied | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? | |
3-fluoropyruvate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? | |
3-methyl-2-oxopentanoate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Neisseria meningitidis | (2S)-2-isopropylmalate + CoA | - |
? | |
alpha-ketoisovalerate + acetyl-CoA + H2O | - |
Neisseria meningitidis | alpha-isopropylmalate + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Neisseria meningitidis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
alpha-IPMS | - |
Neisseria meningitidis |
Alpha-isopropylmalate synthase | - |
Neisseria meningitidis |
NmeIPMS | - |
Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
alpha-ketoisovalerate | pH 7.5, 25°C | Neisseria meningitidis | |
0.28 | - |
3-methyl-2-oxopentanoate | pH 7.5, 25°C | Neisseria meningitidis | |
1.36 | - |
alpha-Ketobutyrate | pH 7.5, 25°C | Neisseria meningitidis | |
1.5 | - |
pyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
105 | - |
3-Fluoropyruvate | pH 7.5, 25°C | Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Neisseria meningitidis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
3,3-dibromopyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
0.06 | - |
3-Bromopyruvate | pH 7.5, 25°C | Neisseria meningitidis | |
2.5 | - |
(S)-alpha-hydroxyisovalerate | pH 7.5, 25°C | Neisseria meningitidis |