Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5',5',5'-trifluoroleucine | - |
Saccharomyces cerevisiae | |
CoA | - |
Saccharomyces cerevisiae | |
D-leucine amide | weak | Saccharomyces cerevisiae | |
L-2-Hydroxyisopentanoate | - |
Saccharomyces cerevisiae | |
leucine | both Fe2+ and Co2+ lower the inhibition by Leu; D-Leu, weak; mixed-type inhibition, strongly pH-dependent, Leu concentration necessary for half-maximal inhibition increases about 10fold as the pH increases from 7.5 to 8.5 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
0.016 | - |
2-oxo-3-methylbutanoate | - |
Saccharomyces cerevisiae | |
0.57 | - |
2-oxobutanoate | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
121000 | - |
in absence of Leu, gel filtration | Saccharomyces cerevisiae |
137000 | - |
in presence of substrate, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Saccharomyces cerevisiae | first enzyme in biosynthesis of L-Leu | ? | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Saccharomyces cerevisiae 60615 | first enzyme in biosynthesis of L-Leu | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae 60615 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.58 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | i.e. alpha-isopropylmalate | ? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae 60615 | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | i.e. alpha-isopropylmalate | ? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Saccharomyces cerevisiae | ? | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Saccharomyces cerevisiae 60615 | ? | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae 60615 | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae 60615 | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Leu, and to a lesser extent, 2-oxo-3-methylbutanoate stabilize against heat inactivation, acetyl-CoA accelerates inactivation | Saccharomyces cerevisiae |
50 | - |
pH 6.9, 5 min, 80% loss of activity, 1 mM Leu provides significant but incomplete protection | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharomyces cerevisiae |