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Literature summary for 2.3.2.5 extracted from

  • Huang, K.F.; Hsu, H.L.; Karim, S.; Wang, A.H.
    Structural and functional analyses of a glutaminyl cyclase from Ixodes scapularis reveal metal-independent catalysis and inhibitor binding (2014), Acta Crystallogr. Sect. D, 70, 789-801.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA the enzymatic activity increases by about 55% in the presence of 1 mM EDTA Ixodes scapularis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells Ixodes scapularis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 10% (w/v) PEG 8000, 8% (v/v) ethylene glycol, 0.1 M HEPES pH 7.5 Ixodes scapularis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Ixodes scapularis
1-Benzylimidazole
-
Ixodes scapularis
dipicolinic acid
-
Ixodes scapularis
Nomega-acetylhistamine
-
Ixodes scapularis
PBD150
-
Ixodes scapularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0218
-
L-glutaminyl 2-naphthylamide at pH 8.0 and 25°C Ixodes scapularis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is metal-independent Ixodes scapularis
Zn2+ 0.5 mol of zinc per mole of enzyme Ixodes scapularis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutaminyl-peptide Ixodes scapularis
-
5-oxoprolyl-peptide + NH3
-
?

Organism

Organism UniProt Comment Textmining
Ixodes scapularis B7QK46
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl S-100 gel filtration Ixodes scapularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutaminyl 2-naphthylamide
-
Ixodes scapularis L-glutamine + 2-naphthylamine
-
?
L-glutaminyl-peptide
-
Ixodes scapularis 5-oxoprolyl-peptide + NH3
-
?

Synonyms

Synonyms Comment Organism
glutaminyl cyclase
-
Ixodes scapularis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
melting temperature Ixodes scapularis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.8
-
L-glutaminyl 2-naphthylamide at pH 8.0 and 25°C Ixodes scapularis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000342
-
PBD150 at pH 8.0 and 25°C Ixodes scapularis
0.00345
-
1,10-phenanthroline at pH 8.0 and 25°C Ixodes scapularis
0.0102
-
1-Benzylimidazole at pH 8.0 and 25°C Ixodes scapularis
0.0142
-
Nomega-acetylhistamine at pH 8.0 and 25°C Ixodes scapularis
0.0368
-
dipicolinic acid at pH 8.0 and 25°C Ixodes scapularis