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Literature summary for 2.3.2.5 extracted from

  • Cornell, H.J.; Doherty, W.; Stelmasiak, T.
    Papaya latex enzymes capable of detoxification of gliadin (2009), Amino Acids, 38, 155-165.
    View publication on PubMed
Search for more literature for 2.3.2.5

Application

Application Comment Organism
drug development QCT would be suitable for enzyme therapy in gluten intolerance and appear to have synergistic action with porcine intestinal extracts, potential for enzyme therapy in coeliac disease Carica papaya

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 fraction are estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography is carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-
Carica papaya O81226
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme is semi-purified from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Carica papaya
-
latex estimated as a fraction of papaya latex Carica papaya
-

Synonyms

Synonyms Comment Organism
glutamine cyclotransferase
-
 Carica papaya
QCT
-
 Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 heat stability of QCT up to 85°C Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/L NaCl Carica papaya