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Literature summary for 2.3.2.16 extracted from

  • Hegde, S.S.; Shrader, T.E.
    FemABX family members are novel nonribosomal peptidyltransferases and important pathogen-specific drug targets (2001), J. Biol. Chem., 276, 6998-7003.
    View publication on PubMed
Search for more literature for 2.3.2.16

Cloned(Commentary)

Cloned (Comment) Organism
-
 Weissella viridescens

Protein Variants

Protein Variants Comment Organism
F196L 26% of wild-type activity Weissella viridescens
F196Y 87% of wild-type activity Weissella viridescens
F305L 16% of wild-type activity Weissella viridescens
F305Y 98% of wild-type activity Weissella viridescens
G292V inactive Weissella viridescens
G319S 13% of wild-type activity Weissella viridescens
G319V 5% of wild-type activity Weissella viridescens
K306A 8% of wild-type activity Weissella viridescens
K306C 38% of wild-type activity Weissella viridescens
K306N 9% of wild-type activity Weissella viridescens
K306R 25% of wild-type activity Weissella viridescens
P110F inactive Weissella viridescens
P110H inactive Weissella viridescens
Q144E 11% of wild-type activity Weissella viridescens
Q144T 79% of wild-type activity Weissella viridescens
Q29E inactive Weissella viridescens
Q29T inactive Weissella viridescens
Y216F 41% of wild-type activity Weissella viridescens
Y216L inactive Weissella viridescens
Y73F 40% of wild-type activity Weissella viridescens
Y73L 40% of wild-type activity Weissella viridescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39360
-
 1 * 39360, calculated Weissella viridescens
40000
-
 gel filtration Weissella viridescens

Organism

Organism UniProt Comment Textmining
Weissella viridescens Q9EY50
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Weissella viridescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycyl-tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-D-Ala addition of serine and alanine is preferred over glycine Weissella viridescens tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-(N6-Gly)Lys-D-Ala-D-Ala
-
 ?
L-alanyl-tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-D-Ala addition of serine and alanine is preferred over glycine Weissella viridescens tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-(N6-L-Ala)Lys-D-Ala-D-Ala
-
 ?
L-seryl-tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-D-Ala addition of serine and alanine is preferred over glycine Weissella viridescens tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-(N6-L-Ser)Lys-D-Ala-D-Ala
-
 ?
additional information Assays with mixtures of alanyl-, seryl-, and glycyl-tRNAs result in mixtures of the expected UDP-MurNAc hexapeptides, but no larger species. FemX adds only the first residue of the interchain peptide Weissella viridescens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39360, calculated Weissella viridescens

Synonyms

Synonyms Comment Organism
FemX
-
 Weissella viridescens