Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.15 extracted from

  • Garcia-Garcia, J.D.; Girard, L.; Hernandez, G.; Saavedra, E.; Pardo, J.P.; Rodriguez-Zavala, J.S.; Encalada, R.; Reyes-Prieto, A.; Mendoza-Cozatl, D.G.; Moreno-Sanchez, R.
    Zn-bis-glutathionate is the best co-substrate of the monomeric phytochelatin synthase from the photosynthetic heavy metal-hyperaccumulator Euglena gracilis (2014), Metallomics, 6, 604-616.
    View publication on PubMed
Search for more literature for 2.3.2.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
EGTA
-
 Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 Cd2+-bis-glutathionate in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis
0.0016
-
 Zn2+-bis-glutathionate in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis
0.0018
-
 Zn2+-bis-glutathionate in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis
0.0025
-
 Cd2+-bis-glutathionate in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ required Euglena gracilis
Zn2+ required Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis A0A075TCK5
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity resin column chromatography, and gel filtration Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Cd2+-bis-glutathionate + [Glu(-Cys)]n-Gly
-
 Euglena gracilis ?
-
 ?
metal-bis-glutathionate + [Glu(-Cys)]n-Gly Zn2+-bis-glutathionate is the best cosubstrate. The order of preference for the Me-GS2 substrate is Zn2+ > Cd2+ > Mn2+ > As5+ > As3+ > Cu2+ > Ag+ Euglena gracilis ?
-
 ?
additional information the enzyme does not use gamma-glutamylcysteine or phytochelatin2 as substrate Euglena gracilis ?
-
 ?
Zn2+-bis-glutathionate + [Glu(-Cys)]n-Gly Zn2+-bis-glutathionate is the best cosubstrate Euglena gracilis ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Euglena gracilis

Synonyms

Synonyms Comment Organism
PCS
-
 Euglena gracilis
phytochelatin synthase
-
 Euglena gracilis

General Information

General Information Comment Organism
physiological function the enzyme confers resistance on Cd2+ stress Euglena gracilis