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Literature summary for 2.3.2.15 extracted from
- HPLC method for the determination of phytochelatin synthase activity specific for soft metal ion chelators (2010), J. Inorg. Biochem., 104, 442-445.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | a HPLC method for the analysis of the activity of phytochelatin synthase is developed | Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET-28b for expression in Escherichia coli BL21 Rosetta DE3 pLysS cells | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Arabidopsis thaliana |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56500 | - |
determined by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9S7Z3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on a Ni2+ affinity column | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.09 | - |
10 ng/ml rAtPCS1 | Arabidopsis thaliana |
| 6.6 | - |
30 ng/ml rAtPCS1 | Arabidopsis thaliana |
| 12.6 | - |
50 ng/ml rAtPCS1 | Arabidopsis thaliana |
| 18.4 | - |
10 ng/ml rAtPCS1, 100 ng/ml bovine serum albumin, BSA | Arabidopsis thaliana |
| 20.9 | - |
1 ng/ml rAtPCS1, 10000 ng/ml bovine serum albumin, BSA | Arabidopsis thaliana |
| 24.9 | - |
100 ng/ml rAtPCS1 | Arabidopsis thaliana |
| 25.9 | - |
200 ng/ml rAtPCS1 | Arabidopsis thaliana |
| 30 | - |
10 ng/ml rAtPCS1, 10000 ng/ml bovine serum albumin, BSA | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtPCS1 | - |
Arabidopsis thaliana |
| PCS1 | - |
Arabidopsis thaliana |
| phytochelatin synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
activity assay | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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