Literature summary for 2.3.2.11 extracted from

Hebecker, S.; Krausze, J.; Hasenkampf, T.; Schneider, J.; Groenewold, M.; Reichelt, J.; Jahn, D.; Heinz, D.W.; Moser, J.
Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine (2015), Proc. Natl. Acad. Sci. USA, 112, 10691-10696.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
catalytic domain, hanging drop vapor diffusion method, using 7.5 mM CoCl2, 85 mM Mes (pH 5.7), 1.53 M (NH4)2SO4, and 15% (v/v) glycerol or 85 mM Na-acetate (pH 6.37), 1.6 M (NH4)2SO4, and 20% (v/v) glycerol	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-alanyl-tRNAAla + phosphatidylglycerol	Pseudomonas aeruginosa	-	tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I537	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose column chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanyl-tRNAAla + phosphatidylglycerol	-	Pseudomonas aeruginosa	tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol	-	?

Synonyms

Synonyms	Comment	Organism
A-PGS	-	Pseudomonas aeruginosa
alanyl-phosphatidylglycerol synthase	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)