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Literature summary for 2.3.2.10 extracted from

  • Plapp, R.; Strominger, J.L.
    Biosynthesis of the peptidoglycan of bacterial cell walls (1970), J. Biol. Chem., 245, 3673-3682.
No PubMed abstract available
Search for more literature for 2.3.2.10

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA or metal ions Weissella viridescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 UDP-acetylmuramoylpentapeptide
-
 Weissella viridescens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Weissella viridescens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by EDTA or metal ions Weissella viridescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 PAGE Weissella viridescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens involved in peptidoglucan metabolism, responsible for interpeptide bridges tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ir

Organism

Organism UniProt Comment Textmining
Weissella viridescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Weissella viridescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
276
-
 purified enzyme Weissella viridescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycyl-tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine poor substrate Weissella viridescens tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(glycyl)-L-lysyl-D-alanyl-D-alanine
-
 ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine poor or no acceptors are acetylmuramoyl- and phosphoacetylmuramoyl-L-Ala-L-Glu-L-Lys-D-Ala-D-Ala or UDP-acetylmuramoyl-L-Ala-L-Glu-L-Lys Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine no strict specificity for tRNA-carrier: tRNACys can replace tRNAAla, tRNA from other species can substitute to some extent for the homologous tRNA from Lactobacillus viridescens, higher specificity for transferred amino acid: amino acids with larger substituents are no substrates, UDP-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala can replace UDP-acetylmuramyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala at 15% the transfer rate Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine involved in peptidoglucan metabolism, responsible for interpeptide bridges Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ir
L-cysteinyl-tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-cysteinyl)-L-lysyl-D-alanyl-D-alanine
-
 ir
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine about half as effective as L-alanyl-tRNA Weissella viridescens tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Weissella viridescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2 L-alanyl-tRNA Weissella viridescens
7.2 7.6 L-seryl-tRNA Weissella viridescens

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.6 L-alanyl-tRNA, about 80% of maximal activity at pH 5.2 and pH 8.6 Weissella viridescens
6.1 8.6 L-seryl-tRNA, about half-maximal activity at pH 6.1 and about 90% of maximal activity at pH 8.6 Weissella viridescens