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Literature summary for 2.3.1.93 extracted from
- A novel O-tigloyltransferase for alkaloid biosynthesis in plants. Purification, characterization, and distribution in Lupinus plants (1994), J. Biol. Chem., 269, 15853-15860.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Lupinus albus |  |
| dithiothreitol | 140% activity at 1 mM | Lupinus albus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (+)-epilupinine | - |
Lupinus albus | |
| (+)-lupanine | - |
Lupinus albus | |
| CoA | competitive | Lupinus albus | |
| N-ethylmaleimide | - |
Lupinus albus | |
| p-chloromercuriphenyl sulfonic acid | - |
Lupinus albus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
13-alpha-hydroxymultiflorine | - |
Lupinus albus | |
| 0.027 | - |
13-hydroxylupanine | - |
Lupinus albus | |
| 0.46 | - |
tigloyl-CoA | with 13-alpha-hydroxymultiflorine | Lupinus albus | |
| 0.52 | - |
tigloyl-CoA | with 13-alpha-hydroxylupanine | Lupinus albus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
isoform A and B, gel filtration | Lupinus albus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tigloyl-CoA + 13-alpha-hydroxymultiflorine | Lupinus albus | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | CoA + 13-tigloyloxymultiflorine | - |
? | |
| tigloyl-CoA + 13-alpha-hydroxymultiflorine | Lupinus albus | isoform A and B | CoA + 13-tigloyloxymultiflorine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | CoA + 13-tigloyloxylupanine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | isoform A and B | CoA + 13-tigloyloxylupanine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | CoA + 13-tigloyloxylupanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cytisus scoparius | - |
- |
- |
| Lupinus albus | - |
bitter and sweet variant | - |
| Lupinus albus | - |
2 isoforms A and B | - |
| Lupinus hirsutus | - |
- |
- |
| Lupinus polyphyllus x Lupinus arboreus | - |
- |
- |
| no activity in Lupinus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lupinus albus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Lupinus albus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
isoform A | Lupinus albus |
| 2.18 | - |
isoform B | Lupinus albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Lupinus albus | ? | - |
? | |
| additional information | substrate specificity, overview | Lupinus albus | ? | - |
? | |
| additional information | baptifoline, epilupinine and lupinine are no substrates | Lupinus albus | ? | - |
? | |
| tigloyl-CoA + 13-alpha-hydroxymultiflorine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus albus | CoA + 13-tigloyloxymultiflorine | - |
? | |
| tigloyl-CoA + 13-alpha-hydroxymultiflorine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus hirsutus | CoA + 13-tigloyloxymultiflorine | - |
? | |
| tigloyl-CoA + 13-alpha-hydroxymultiflorine | isoform A and B | Lupinus albus | CoA + 13-tigloyloxymultiflorine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Cytisus scoparius | CoA + 13-tigloyloxylupanine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus polyphyllus x Lupinus arboreus | CoA + 13-tigloyloxylupanine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | isoform A and B | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? | |
| tigloyl-CoA + 13-hydroxylupanine | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, isoform A and B, SDS-PAGE | Lupinus albus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tigloyl:13alpha-hydroxylupanine O-tigloyltransferase | HLTase | Lupinus albus |
| tigloyl:13alpha-hydroxymultiflorine O-tigloyltransferase | HMTase | Lupinus albus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
5 min, complete inactivation | Lupinus albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
isoform 1 pI: 7.8, isoform 2 pI: 7.6 | Lupinus albus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
CoA | - |
Lupinus albus | |
| 1.7 | - |
(+)-lupanine | - |
Lupinus albus | |
| 3.1 | - |
(+)-epilupinine | - |
Lupinus albus | |
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