Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates about 50-80% | Lupinus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldithiocarbamate | - |
Lupinus albus | |
HgCl2 | strong | Lupinus albus | |
iodoacetamide | slight inhibition | Lupinus albus | |
additional information | EDTA is no inhibitor | Lupinus albus | |
N-ethylmaleimide | strong | Lupinus albus | |
p-hydroxymercuribenzoate | strong | Lupinus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
13-hydroxylupanine | - |
Lupinus albus | |
0.14 | - |
tigloyl-CoA | - |
Lupinus albus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | cations, e.g. Fe2+, Ca2+, Mg2+, NH4Cl, Fe3+ have no significant influence | Lupinus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | Lupinus polyphyllus | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | Lupinus polyphyllus | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | CoA + 13-tigloyloxylupanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus albus | - |
- |
- |
Lupinus polyphyllus | - |
- |
- |
no activity in Conium maculatum | - |
- |
- |
no activity in Cytisus scoparius | - |
- |
- |
no activity in Pisum sativum | - |
- |
- |
no activity in Vicia faba | - |
- |
- |
Oxidation Stability | Organism |
---|---|
O2-sensitive, dithioerythritol stabilizes | Lupinus albus |
Purification (Comment) | Organism |
---|---|
partial | Lupinus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | leaflet | Lupinus polyphyllus | - |
additional information | not in root and cell suspension culture | Lupinus polyphyllus | - |
petiole | - |
Lupinus polyphyllus | - |
root | - |
Lupinus albus | - |
seedling | high activity in stem and root | Lupinus albus | - |
stem | - |
Lupinus polyphyllus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylbutyryl-CoA + 13-hydroxylupanine | 97% of activity compared to tigloyl-CoA | Lupinus albus | CoA + 13-(3-methylbutyryl)oxylupanine | - |
ir | |
benzoyl-CoA + 13-hydroxylupanine | 111% of activity compared to tigloyl-CoA | Lupinus albus | CoA + 13-benzoyloxylupanine | - |
ir | |
butyryl-CoA + 13-hydroxylupanine | 27% of activity compared to tigloyl-CoA | Lupinus albus | CoA + 13-butyryloxylupanine | - |
ir | |
additional information | lupinine, 4-hydroxylupinine, and cholesterol and acetyl-CoA are no substrates | Lupinus albus | ? | - |
? | |
propionyl-CoA + 13-hydroxylupanine | 25% of activity compared to tigloyl-CoA | Lupinus albus | CoA + 13-propionyloxylupanine | - |
ir | |
tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
ir | |
tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus polyphyllus | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | Lupinus polyphyllus | CoA + 13-tigloyloxylupanine | - |
? | |
valeryl-CoA + 13-hydroxylupanine | 73% of activity compared to tigloyl-CoA | Lupinus albus | CoA + 13-valeryloxylupanine | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lupinus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Lupinus albus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | half-maximal activity at pH 6.5, maximal activity at pH 8.0 | Lupinus albus |