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Literature summary for 2.3.1.92 extracted from

  • Gräwe, W.; Bachhuber, P.; Mock, H.P.; Strack, D.
    Purification and characterization of sinapoylglucose:malate sinapoyltransferase from Raphanus sativus L. (1992), Planta, 187, 236-241.
    View publication on PubMed
Search for more literature for 2.3.1.92

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by dithioerythritol and 2-mercaptoethanol Raphanus sativus

General Stability

General Stability Organism
1 mg/ml bovine serum albumin stabilizes Raphanus sativus
L-malate stabilizes during purification Raphanus sativus
thawing after freezing at -20°C leads to 50% loss of activity, bovine serum albumin stabilizes Raphanus sativus
Unstable to dilution Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
D-malate non-competitive Raphanus sativus
L-malate above 0.05 M Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Raphanus sativus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Raphanus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 1 * 51000, SDS-PAGE Raphanus sativus
52000
-
 gel filtration Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
 cv. Saxa Nova
-
Raphanus sativus
-
 two isoforms
-
Raphanus sativus
-
 red radish, var. sativus
-

Purification (Commentary)

Purification (Comment) Organism
2 isozymes on isoelectric focusing of purified enzyme, 4 isoforms on isoelectric focusing of crude extract, in varying proportions depending on light conditions during growth Raphanus sativus

Reaction

Reaction Comment Organism Reaction ID
1-O-sinapoyl-beta-D-glucose + (S)-malate = D-glucose + sinapoyl-(S)-malate mechanism Raphanus sativus
a

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.528
-
-
 Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-di-O-sinapoyl-beta-D-glucose + L-malate at 26% the rate of the reaction with 1-O-sinapoylglucose Raphanus sativus ?
-
 ?
1-O-(4-coumaroyl)-beta-D-glucose + L-malate poor substrate Raphanus sativus D-glucose + 4-coumaroyl-L-malate
-
 ?
1-O-caffeoyl-beta-D-glucose + L-malate at 45% the rate of the reaction with 1-O-sinapoylglucose Raphanus sativus D-glucose + caffeoyl-L-malate
-
 ?
1-O-feruloyl-beta-D-glucose + L-malate at 85% the rate of the reaction with 1-O-sinapoylglucose Raphanus sativus D-glucose + feruloyl-L-malate
-
 ?
1-O-sinapoyl-beta-D-glucose + L-malate
-
 Raphanus sativus D-glucose + sinapoyl-L-malate
-
 ir

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Raphanus sativus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.5 about half-maximal activity at pH 5.5 and 6.5 Raphanus sativus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
215
-
 D-malate
-
 Raphanus sativus