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Literature summary for 2.3.1.9 extracted from

  • Ramos-Vera, W.H.; Weiss, M.; Strittmatter, E.; Kockelkorn, D.; Fuchs, G.
    Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota (2011), J. Bacteriol., 193, 1201-1211.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as soluble N-terminal His10-tagged protein in Escherichia coli Pyrobaculum neutrophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 acetoacetyl-CoA pH 7.8, 85°C Pyrobaculum neutrophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42444
-
 x * 42444, calculated from sequence Pyrobaculum neutrophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Pyrobaculum neutrophilum the enzyme is involved in autotrophic carbon fixation 2 acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA Pyrobaculum neutrophilum DSM 2338 the enzyme is involved in autotrophic carbon fixation 2 acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrobaculum neutrophilum B1YB71
-
-
Pyrobaculum neutrophilum DSM 2338 B1YB71
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrobaculum neutrophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55
-
 pH 7.2, 85°C Pyrobaculum neutrophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
 Pyrobaculum neutrophilum 2 acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA the enzyme is involved in autotrophic carbon fixation Pyrobaculum neutrophilum 2 acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA
-
 Pyrobaculum neutrophilum DSM 2338 2 acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA the enzyme is involved in autotrophic carbon fixation Pyrobaculum neutrophilum DSM 2338 2 acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 42444, calculated from sequence Pyrobaculum neutrophilum

Synonyms

Synonyms Comment Organism
beta-ketothiolase
-
 Pyrobaculum neutrophilum
Tneu_0249 locus name Pyrobaculum neutrophilum

General Information

General Information Comment Organism
physiological function the enzyme is involved in autotrophic carbon fixation Pyrobaculum neutrophilum