Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Escherichia coli | Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
acetoacetyl-CoA | pH 7.5, 70°C | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | Metallosphaera sedula | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | 2 acetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | Metallosphaera sedula DSM 5348 | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | 2 acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YEH9 | - |
- |
Metallosphaera sedula DSM 5348 | A4YEH9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | - |
Metallosphaera sedula | 2 acetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | Metallosphaera sedula | 2 acetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | - |
Metallosphaera sedula DSM 5348 | 2 acetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | Metallosphaera sedula DSM 5348 | 2 acetyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Msed_0656 | locus name | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1000 | - |
acetoacetyl-CoA | pH 7.5, 70°C | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Metallosphaera sedula |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | Metallosphaera sedula |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5600 | - |
acetoacetyl-CoA | pH 7.5, 70°C | Metallosphaera sedula |