Application | Comment | Organism |
---|---|---|
synthesis | the enzyme has biotechnological potential in haloarchaea for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with controllable 3-oxopentano content, from unrelated cheap carbon sources | Haloferax mediterranei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the deletion mutant strain expressing the enzyme with an S83A, H281Q, or H331Q mutation in BktBalpha does not accumulate polyhydroxyalkanoate at all, whereas strains expressing the enzyme with a C365A or C365S mutation in subunit BktBalpha have no significant effect on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation | Haloferax mediterranei |
additional information | the introduction of the S84A, H282Q, or H332Q mutation into subunit PhaAalpha results in inactive enzyme, while the C366A or C366S mutation does not obviously affect PhaA activity | Haloferax mediterranei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 acetyl-CoA | Haloferax mediterranei | the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | CoA + acetoacetyl-CoA | - |
? | |
2 acetyl-CoA | Haloferax mediterranei DSM 1411 | the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | CoA + acetoacetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | I3R3D1 and I3R3D0 | I3R3D1: catalytic subunit PhaAalpha, I3RA71: subunit PhaAbeta | - |
Haloferax mediterranei | I3RA72 and I3RA71 | I3RA72: catalytic subunit BktBalpha, I3RA71: subunit BktBbeta | - |
Haloferax mediterranei DSM 1411 | I3R3D1 and I3R3D0 | I3R3D1: catalytic subunit PhaAalpha, I3RA71: subunit PhaAbeta | - |
Haloferax mediterranei DSM 1411 | I3RA72 and I3RA71 | I3RA72: catalytic subunit BktBalpha, I3RA71: subunit BktBbeta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 acetyl-CoA | - |
Haloferax mediterranei | CoA + acetoacetyl-CoA | - |
? | |
2 acetyl-CoA | the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | Haloferax mediterranei | CoA + acetoacetyl-CoA | - |
? | |
2 acetyl-CoA | - |
Haloferax mediterranei DSM 1411 | CoA + acetoacetyl-CoA | - |
? | |
2 acetyl-CoA | the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | Haloferax mediterranei DSM 1411 | CoA + acetoacetyl-CoA | - |
? | |
acetyl-CoA + propionyl-CoA | - |
Haloferax mediterranei | CoA + 3-oxopentanoyl-CoA | - |
? | |
acetyl-CoA + propionyl-CoA | - |
Haloferax mediterranei DSM 1411 | CoA + 3-oxopentanoyl-CoA | - |
? | |
additional information | the catalytic triad of BktBalpha is likely to be Ser-His-His | Haloferax mediterranei | ? | - |
? | |
additional information | the catalytic triad of PhaAalpha is likely to be Ser-His-His | Haloferax mediterranei | ? | - |
? | |
additional information | the catalytic triad of PhaAalpha is likely to be Ser-His-His | Haloferax mediterranei DSM 1411 | ? | - |
? | |
additional information | the catalytic triad of BktBalpha is likely to be Ser-His-His | Haloferax mediterranei DSM 1411 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | composed of two different types of subunits: catalytic subunit PhaAalpha, and subunit PhaABbeta | Haloferax mediterranei |
More | composed of two different types of subunits: catalytic subunit BktBalpha, and BktBbeta | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
HFX_1022 | locus name, subunit PhaAbeta | Haloferax mediterranei |
HFX_1023 | locus name, subunit PhaAalpha | Haloferax mediterranei |
HFX_6003 | locus name, subunit BktBbeta | Haloferax mediterranei |
HFX_6004 | locus name, subunit BktBalpha | Haloferax mediterranei |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | Haloferax mediterranei |