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Literature summary for 2.3.1.9 extracted from

  • Tanaka, T.; Shima, Y.; Ogawa, N.; Nagayama, K.; Yoshida, T.; Ohmachi, T.
    Expression, identification and purification of Dictyostelium acetoacetyl-CoA thiolase expressed in Escherichia coli (2011), Int. J. Biol. Sci., 7, 9-17.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43445, calculated, x * 43000, SDS-PAGE Dictyostelium discoideum
43445
-
 x * 43445, calculated, x * 43000, SDS-PAGE Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum Q86AD9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Dictyostelium discoideum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
 30°C, pH 8.3 Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + CoA
-
 Dictyostelium discoideum 2 acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 43445, calculated, x * 43000, SDS-PAGE Dictyostelium discoideum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to Dictyostelium discoideum
60
-
 10 min, 50% residual activity Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Dictyostelium discoideum

pH Range

pH Minimum pH Maximum Comment Organism
7 9 significant decrease in activiy below pH 7.0 or above pH 9.0 Dictyostelium discoideum

pI Value

Organism Comment pI Value Maximum pI Value
Dictyostelium discoideum calculated
-
 8.4