Cloned (Comment) | Organism |
---|---|
gene mmgA, encoded in the mmg operon, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
acetoacetyl-CoA | pH 6.0, 37°C | Bacillus subtilis | |
0.07 | - |
CoA | pH 6.0, 37°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, recombinant His-tagged enzyme, SDS-PAGE | Bacillus subtilis |
84000 | - |
about, recombinant His-tagged enzyme, gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene mmgA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + CoA | the enzyme shows preference for the thiolytic reaction direction | Bacillus subtilis | 2 acetyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, recombinant His-tagged enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
acetoacetyl-CoA thiolase | - |
Bacillus subtilis |
MmgA | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
acetoacetyl-CoA | pH 6.0, 37°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Bacillus subtilis |