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Literature summary for 2.3.1.9 extracted from

  • Liu, T.; Liu, S.J.; Xue, Y.; Ma, Y.; Zhou, P.
    Purification and characterization of an extremely halophilic acetoacetyl-CoA thiolase from a newly isolated Halobacterium strain ZP-6 (2002), Extremophiles, 6, 97-102.
    View publication on PubMed
Search for more literature for 2.3.1.9

Activating Compound

Activating Compound Comment Organism Structure
KCl maximal activity at 4.5 M Halobacterium sp.
NaCl maximal activity at 4.5 M Halobacterium sp.

General Stability

General Stability Organism
more than 10% sucrose, 2-mercaptoethanol or dithiothreitol are necessary to maintain activity Halobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, CTAB-PAGE Halobacterium sp.
60000
-
 2 * 60000, SDS-PAGE Halobacterium sp.
80000
-
 gel filtration Halobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Halobacterium sp.
-
 acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA Halobacterium sp. ZP-6
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Halobacterium sp.
-
 haloarchaeon
-
Halobacterium sp. ZP-6
-
 haloarchaeon
-

Purification (Commentary)

Purification (Comment) Organism
poly ethylene glycol 6000, DEAE-cellulose 52, benzyl-Sepharose, hydroxylapatite Halobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
833.3
-
-
 Halobacterium sp.

Storage Stability

Storage Stability Organism
25°C, 1 week, 4.5 M KCl, no loss of activity Halobacterium sp.
25°C, 72 h, 200 mM KCl, 90% loss of activity Halobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
 Halobacterium sp. acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA
-
 Halobacterium sp. acetyl-CoA + acetyl-CoA
-
 r
CoA + acetoacetyl-CoA
-
 Halobacterium sp. ZP-6 acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA
-
 Halobacterium sp. ZP-6 acetyl-CoA + acetyl-CoA
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE Halobacterium sp.
dimer 2 * 43000, CTAB-PAGE Halobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 1 h, complete loss of activity Halobacterium sp.
70
-
 10 h, 4 M KCl, 20% loss of activity Halobacterium sp.
85
-
 10 h, 4 M KCl, 35% loss of activity Halobacterium sp.