KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
CoA | peroxisomal thiolase, thiolysis | Rattus norvegicus | |
0.009 | - |
acetoacetyl-CoA | peroxisomal thiolase, thiolysis | Rattus norvegicus | |
0.016 | - |
acetoacetyl-CoA | cytosolic thiolase, thiolysis | Rattus norvegicus | |
0.02 | - |
CoA | cytosolic thiolase, thiolysis | Rattus norvegicus | |
0.035 | - |
acetoacetyl-CoA | peroxisomal thiolase, synthesis | Rattus norvegicus | |
0.08 | - |
acetoacetyl-CoA | cytosolic thiolase, synthesis | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Rattus norvegicus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, peroxisomal thiolase, SDS-PAGE | Rattus norvegicus |
160000 | - |
peroxisomal thiolase, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | Rattus norvegicus | peroxisomal thiolase most probably catalyzes the first reaction in peroxisomal cholesterol and dolichol synthesis | acetyl-CoA + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
peroxisomal thiolase, DEAE-cellulose, phosphocellulose column, Blue-Sepharose | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
100 | 130 | peroxisomal thiolase, thiolysis | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | - |
Rattus norvegicus | acetyl-CoA + acetyl-CoA | - |
r | |
CoA + acetoacetyl-CoA | peroxisomal thiolase most probably catalyzes the first reaction in peroxisomal cholesterol and dolichol synthesis | Rattus norvegicus | acetyl-CoA + acetyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, peroxisomal thiolase, SDS-PAGE | Rattus norvegicus |