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Literature summary for 2.3.1.9 extracted from

  • Kim, S.A.; Copeland, L.
    Acetyl coenzyme A acetyltransferase of Rhizobium sp. (Cicer) strain CC 1192 (1997), Appl. Environ. Microbiol., 63, 3432-3437.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.9

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 and 25 mM, 25 and 50% inhibition of thiolysis Rhizobium sp.
CoA substrate inhibition above 0.05 mM, 50% inhibition of synthesis at 0.06 mM in bacteroids Rhizobium sp.
Mg2+ 2 and 5 mM, 20 and 40% inhibition of synthesis, respectively Rhizobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 acetoacetyl-CoA thiolysis Rhizobium sp.
0.056
-
 CoA thiolysis Rhizobium sp.
0.38
-
 acetyl-CoA enzyme from bacteroids Rhizobium sp.
1.06
-
 acetyl-CoA synthesis Rhizobium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can replace Mg2+, 5 mM, 90% of activity with Mg2+, inhibition above 10 mM Rhizobium sp.
Mg2+ maximal activity at 5-10 mM, inhibition above Rhizobium sp.
Mn2+ can replace Mg2+, 5 mM, 90% of activity with Mg2+, inhibition above Rhizobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 4 * 44000, SDS-PAGE Rhizobium sp.
180000
-
 gel filtration Rhizobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Rhizobium sp.
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizobium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Econo-Q, Superose 6, Mono Q, HA-Ultrogel, Mono Q Rhizobium sp.

Source Tissue

Source Tissue Comment Organism Textmining
bacteroid
-
 Rhizobium sp.
-
free-living state
-
 Rhizobium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
163
-
-
 Rhizobium sp.

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM 1 mM, EDTA, 15 d, loss of more than 50% activity Rhizobium sp.
-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM dithiothreitol, 1 mM, EDTA, 15 d, loss of 20% activity Rhizobium sp.
-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM dithiothreitol, 1 mM, EDTA, 20% glycerol, 60 d, loss of less than 20% activity Rhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
 Rhizobium sp. acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA
-
 Rhizobium sp. acetyl-CoA + acetyl-CoA
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE Rhizobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 thiolysis, more than 90% of maximal activity between pH 7.5 and pH 8.3 Rhizobium sp.
8.1
-
 synthesis, more than 90% of maximal activity between pH 7.5 and pH 8.5 Rhizobium sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.006
-
 CoA enzyme from bacteroids Rhizobium sp.
0.011
-
 CoA
-
 Rhizobium sp.