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Literature summary for 2.3.1.9 extracted from
- On the mechanism of ketogenesis and its control. Purification, kinetic mechanism and regulation of different forms of mitochondrial acetoacetyl-CoA thiolases from ox liver (1975), Eur. J. Biochem., 59, 475-489.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetyl-CoA | - |
Bos taurus |  |
| CoA | both directions | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
acetoacetyl-CoA | isoenzyme A, thiolysis | Bos taurus | |
| 0.025 | - |
CoA | isoenzyme A, thiolysis | Bos taurus | |
| 0.032 | - |
acetoacetyl-CoA | isoenzyme B, thiolysis | Bos taurus | |
| 0.08 | - |
acetyl-CoA | enzyme B | Bos taurus | |
| 0.091 | - |
acetyl-CoA | isoenzyme A, synthesis | Bos taurus | |
| 0.091 | - |
acetyl-CoA | isoenzyme B, synthesis | Bos taurus | |
| 0.154 | - |
CoA | isoenzyme B, thiolysis | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | Bos taurus | - |
acetyl-CoA + acetyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
2 forms: A and B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes A and B, ammonium sulfate, cellulose phosphate column, crystallization | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 acetyl-CoA = CoA + acetoacetyl-CoA | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, less than 0.2 mg/ml protein, 50 mM Tris, pH 7.2, 0.5 mM dithiothreitol, 8 d, 60-70% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | - |
Bos taurus | acetyl-CoA + acetyl-CoA | - |
? | |
| CoA + acetoacetyl-CoA | rate of synthesis is 0.31 and 0.08 the rate of thiolysis for isoenzyme A and B, respectively | Bos taurus | acetyl-CoA + acetyl-CoA | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | 8.4 | isoenzyme A and B, acetoacetyl-CoA synthesis | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
acetoacetyl-CoA | isoenzyme A | Bos taurus | |
| 0.05 | - |
CoA | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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