Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of a didomain subconstruct KS-MAT (2?853), bearing a beto-ketoacyl synthase KS knockout C161G and malonyl/acetyltransferase MAT | Mus musculus |
R606A | active site mutant of didomain construct. The specificity constant for malonyl-CoA is 40fold smaller than the wild-type constant | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00062 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00073 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00086 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00096 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00128 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00136 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00163 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00164 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00172 | - |
3-hydroxybutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.00206 | - |
3-methylbutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.0021 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.003 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
0.0048 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
0.018 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P19096 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybutanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + 3-hydroxybutanoyl-[acyl-carrier protein] | - |
? | |
3-methylbutanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + 3-methylbutanoyl-[acyl-carrier protein] | - |
? | |
acetoacetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + acetoacetyl-[acyl-carrier protein] | - |
? | |
acetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + acetyl-[acyl-carrier protein] | - |
? | |
butanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + butanoyl-[acyl-carrier protein] | - |
? | |
crotonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + crotonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + a malonyl-[acyl-carrier protein] | - |
? | |
methylmalonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + a methylmalonyl-[acyl-carrier protein] | - |
? | |
octanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + octanoyl-[acyl-carrier protein] | - |
? | |
palmitoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + palmitoyl-[acyl-carrier protein] | - |
? | |
phenylacetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + phenylacetyl-[acyl-carrier protein] | - |
? | |
succinyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + succinyl-[acyl-carrier protein] | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
4 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
4.1 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
5 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
6 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
6 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
6.1 | - |
3-hydroxybutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
7.2 | - |
3-methylbutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
7.3 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
10.8 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
13 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
13.4 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
13.7 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
15.6 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
300 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
500 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
4000 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
4400 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
5600 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
6200 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
6400 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
6500 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
6600 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
8400 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
8500 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
12200 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus |