Cloned (Comment) | Organism |
---|---|
transacyclase domain | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R606A | reduced malonyl/acetyltransferase activity, increased transacylase activity, 16000fold increased selectivity for acetyl-CoA, 8.5fold increase of Km for malonyl-CoA | Rattus norvegicus |
R606K | reduced malonyl/acetyltransferase activity, increased transacylase activity, 16fold increased selectivity for acetyl-CoA | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
malonyl-CoA | R606K mutant | Rattus norvegicus | |
0.0018 | - |
acetyl-CoA | R606A mutant | Rattus norvegicus | |
0.0019 | - |
malonyl-CoA | wild-type enzyme | Rattus norvegicus | |
0.0029 | - |
acetyl-CoA | R606K mutant | Rattus norvegicus | |
0.0039 | - |
acetyl-CoA | wild-type enzyme | Rattus norvegicus | |
0.0162 | - |
malonyl-CoA | R606A mutant | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
wild-type and mutant | - |
Purification (Comment) | Organism |
---|---|
95% pure, transacyclase domain | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
for malonyl-CoA, R606A mutant | Rattus norvegicus |
0.154 | - |
for malonyl-CoA, R606K mutant | Rattus norvegicus |
1.61 | - |
for malonyl-CoA, wild-type enzyme | Rattus norvegicus |
2.68 | - |
for acetyl-CoA, wild-type enzyme | Rattus norvegicus |
4.53 | - |
for acetyl-CoA, R606K mutant | Rattus norvegicus |
17.8 | - |
for acetyl-CoA, R606A mutant | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
malonyl-CoA | R606K mutant | Rattus norvegicus | |
0.00167 | - |
malonyl-CoA | R606A mutant | Rattus norvegicus | |
0.02 | - |
malonyl-CoA | wild-type enzyme | Rattus norvegicus | |
0.0317 | - |
acetyl-CoA | wild-type enzyme | Rattus norvegicus | |
0.0567 | - |
acetyl-CoA | R606K mutant | Rattus norvegicus | |
0.237 | - |
acetyl-CoA | R606A mutant | Rattus norvegicus |