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Literature summary for 2.3.1.82 extracted from

  • Vetting, M.W.; Park, C.H.; Hegde, S.S.; Jacoby, G.A.; Hooper, D.C.; Blanchard, J.S.
    Mechanistic and structural analysis of aminoglycoside N-acetyltransferase AAC(6)-Ib and its bifunctional, fluoroquinolone-active AAC(6)-Ib-cr variant (2008), Biochemistry, 47, 9825-9835.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-28a+ for expression in Escherichia coli BL21DE3 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure of AAC(6')-Ib is determined in various complexes with donor and acceptor ligands to resolutions greater than 2.2 A Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
lividomycin A
-
 Escherichia coli
Pefloxacin
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00027
-
 kanamycin B AAC(6')-Ib, wild type Escherichia coli
0.0053
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli
0.0067
-
 kanamycin B AAC(6')-Ib-cr variant Escherichia coli
0.02
-
 neomycin B AAC(6')-Ib, wild type Escherichia coli
0.035
-
 acetyl-CoA AAC(6')-Ib, wild type Escherichia coli
0.067
-
 Norfloxacin AAC(6')-Ib-cr variant Escherichia coli
0.07
-
 Ciprofloxacin AAC(6')-Ib-cr variant Escherichia coli
0.108
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on a Ni-NTA and a Superdex S-75 column, the His6 tag is cleaved using thrombin Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + ciprofloxacin
-
 Escherichia coli CoA + N4'-acetylnorfloxacin
-
 ?
acetyl-CoA + kanamycin B
-
 Escherichia coli CoA + N6'-acetylkanamycin B
-
 ?
acetyl-CoA + neomycin B
-
 Escherichia coli CoA + N6'-acetylneomycin B
-
 ?
acetyl-CoA + norfloxacin
-
 Escherichia coli CoA + N4'-acetylnorfloxacin
-
 ?

Synonyms

Synonyms Comment Organism
AAC(6')-Ib
-
 Escherichia coli
AAC(6')-Ib-cr bifunctional variant of AAC(6')-Ib Escherichia coli
aminoglycoside N-acetyltransferase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.03
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli
1.03
-
 Ciprofloxacin AAC(6')-Ib-cr variant Escherichia coli
1.08
-
 Norfloxacin AAC(6')-Ib-cr variant Escherichia coli
2.27
-
 kanamycin B AAC(6')-Ib, wild type Escherichia coli
2.32
-
 acetyl-CoA AAC(6')-Ib, wild type Escherichia coli
3.08
-
 neomycin B AAC(6')-Ib, wild type Escherichia coli
5.23
-
 acetyl-CoA AAC(6')-Ib-cr variant Escherichia coli
5.25
-
 kanamycin B AAC(6')-Ib-cr variant Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 AAC(6')-Ib Escherichia coli

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Escherichia coli