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Literature summary for 2.3.1.82 extracted from

  • Magnet, S.; Lambert, T.; Courvalin, P.; Blanchard, J.S.
    Kinetic and mutagenic characterization of the chromosomally encoded Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase (2001), Biochemistry, 40, 3700-3709.
    View publication on PubMed
Search for more literature for 2.3.1.82

Cloned(Commentary)

Cloned (Comment) Organism
-
 Salmonella enterica

Protein Variants

Protein Variants Comment Organism
C109A mutation neither abolishes activity nor alters the biphasic inactivation by iodoacetamide Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
dithio-CoA
-
 Salmonella enterica
iodoacetamide inactivation in a biphasic manner, half of the activity is lost rapidly and the other half more slowly, tobramycin but not acetyl-CoA protects Salmonella enterica
lividomycin
-
 Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 netilmicin mutant enzyme C109A Salmonella enterica
0.008
-
 netilmicin wild-type enzyme Salmonella enterica
0.01
-
 acetyl-CoA reaction with tobramycin Salmonella enterica
0.012
-
 sisomicin wild-type enzyme Salmonella enterica
0.014
-
 neomycin C wild-type enzyme Salmonella enterica
0.017
-
 kanamycin B mutant enzyme C109A Salmonella enterica
0.017
-
 acetyl-CoA reaction with tobramycin, mutant enzyme C109A Salmonella enterica
0.026
-
 tobramycin mutant enzyme C109A Salmonella enterica
0.03
-
 dibekacin wild-type enzyme Salmonella enterica
0.05
-
 amikacin wild-type enzyme Salmonella enterica
0.05
-
 ribostamycin wild-type enzyme Salmonella enterica
0.066
-
 tobramycin wild-type enzyme Salmonella enterica
0.07
-
 acetyl-CoA reaction with tobramycin, mutant enzyme C109A/C70A Salmonella enterica
0.075
-
 amikacin mutant enzyme C109A Salmonella enterica
0.079
-
 kanamycin B wild-type enzyme Salmonella enterica
0.105
-
 kanamycin A wild-type enzyme Salmonella enterica
0.25
-
 neomycin C mutant enzyme C109A/C70A Salmonella enterica
0.27
-
 propionyl-CoA reaction with tobramycin, wild-type enzyme Salmonella enterica
0.46
-
 netilmicin mutant enzyme C109A/C70A Salmonella enterica
0.53
-
 neomycin A
-
 Salmonella enterica
0.54
-
 tobramycin mutant enzyme C109A/C70A Salmonella enterica
0.82
-
 malonyl-CoA reaction with tobramycin, wild-type enzyme Salmonella enterica
0.9
-
 kanamycin B mutant enzyme C109A/C70A Salmonella enterica
9.3
-
 butyryl-CoA reaction with tobramycin, wild-type enzyme Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 2 * 17000, SDS-PAGE Salmonella enterica
17173
-
 2 * 17173, calculation from nucleotide sequence Salmonella enterica
17184
-
 2 * 17184, electrospray ionization mass spectrometry Salmonella enterica
35000
-
 gel filtration Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Salmonella enterica involved in resistance to antibiotics ?
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + amikacin
-
 Salmonella enterica CoA + N6'-acetylamikacin
-
 ?
acetyl-CoA + dibekacin
-
 Salmonella enterica CoA + N6'-acetyldibekacin
-
 ?
acetyl-CoA + kanamycin A
-
 Salmonella enterica CoA + N6'-acetylkanamycin A
-
 ?
acetyl-CoA + kanamycin B
-
 Salmonella enterica CoA + N6'-acetylkanamycin B
-
 ?
acetyl-CoA + neomycin A
-
 Salmonella enterica CoA + N6'-acetylneomycin A
-
 ?
acetyl-CoA + neomycin C
-
 Salmonella enterica CoA + N6'-acetylneomycin C
-
 ?
acetyl-CoA + netilmicin
-
 Salmonella enterica CoA + N6'-acetylnetilmicin
-
 ?
acetyl-CoA + ribostamycin
-
 Salmonella enterica CoA + N6'-acetylribostamycin
-
 ?
acetyl-CoA + sisomicin
-
 Salmonella enterica CoA + N6'-acetylsisomicin
-
 ?
acetyl-CoA + tobramycin
-
 Salmonella enterica CoA + N6'-acetyltobramycin
-
 ?
butyryl-CoA + tobramycin
-
 Salmonella enterica CoA + N6'-butyryltobramycin
-
 ?
malonyl-CoA + tobramycin
-
 Salmonella enterica CoA + N6'-malonyltobramycin
-
 ?
additional information involved in resistance to antibiotics Salmonella enterica ?
-
 ?
propionyl-CoA + tobramycin tobramycin exhibits a rapid, tobramycin-independent rate of hydrolysis that is linearly proportional to enzyme Salmonella enterica CoA + N6'-propionyltobramycin
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 17000, SDS-PAGE Salmonella enterica
dimer 2 * 17173, calculation from nucleotide sequence Salmonella enterica
dimer 2 * 17184, electrospray ionization mass spectrometry Salmonella enterica