Protein Variants | Comment | Organism |
---|---|---|
C109A/C70A | mutant enzyme is not inactivated by iodoacetamide. Double mutant exhibits large increases in Km-values for both acetyl-CoA and aminoglycoside substrates | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Salmonella enterica | involved in resistance to antibiotics | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
wild-type enzyme and mutant enzymes C109A and C109A/C70A | - |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
equilibrium binding and the directly determined thermodynamic parameters for different aminoglycosides and acyl-CoA derivatives to the wild-type enzyme and two mutant enzymes c109A and C109A/C70A using fluorescence spectroscopy and isothermal titration calorimetry | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | C70 is directly involved in aminoglycoside binding | Salmonella enterica | ? | - |
? | |
additional information | involved in resistance to antibiotics | Salmonella enterica | ? | - |
? |