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Literature summary for 2.3.1.71 extracted from
- Expression, purification, and characterization of mouse glycine N-acyltransferase in Escherichia coli (2014), Protein Expr. Purif., 97, 23-28.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycolic acid | - |
Mus musculus |  |
| oleoyl-CoA | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0094 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 6.1 | - |
glycine | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 9.7 | - |
glycine ethyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 29 | - |
Glycine methyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, calculated | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q91XE0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-CoA + glycine | glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate | Mus musculus | CoA + N-benzoylglycine | - |
? | |
| benzoyl-CoA + glycine ethyl ester | - |
Mus musculus | CoA + N-benzoylglycine ethyl ester | - |
? | |
| benzoyl-CoA + glycine methyl ester | - |
Mus musculus | CoA + N-benzoylglycine methyl ester | - |
? | |
| additional information | enzyme also shows glycine N-acyltransferase activity, reaction of EC 2.3.1.13. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, calculated | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlyAT | - |
Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
glycine ethyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 1 | - |
Glycine methyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 3.2 | - |
glycine | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 4.2 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
pH 8.0, temperature not specified in the publication | Mus musculus | oleoyl-CoA | |
| 58 | - |
pH 8.0, temperature not specified in the publication | Mus musculus | glycolic acid | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
Glycine methyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 0.059 | - |
glycine ethyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 0.52 | - |
glycine | pH 8.0, temperature not specified in the publication | Mus musculus | |
| 450 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
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