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Literature summary for 2.3.1.71 extracted from

  • Van der Sluis, R.; Badenhorst, C.; Van der Westhuizen, F.; Van Dijk, A.
    Characterisation of the influence of genetic variations on the enzyme activity of a recombinant human glycine N-acyltransferase (2013), Gene, 515, 447-453.
    View publication on PubMed
Search for more literature for 2.3.1.71

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular model containing coenzyme A. The inactivity of the R199C variant could be due to the substitution of the highly conserved Arg199 and destabilisation of an alpha-loop-alpha motif which is important for substrate binding Homo sapiens

Protein Variants

Protein Variants Comment Organism
E229Q single nucleotide polymorphism, inactive Homo sapiens
F168L single nucleotide polymorphism, decreased activity compared to wild-type Homo sapiens
K16N single nucleotide polymorphism, enzymic activities similar to wild-type Homo sapiens
N156S single nucleotide polymorphism, more active than wild-type Homo sapiens
R131H single nucleotide polymorphism, enzymic activities similar to wild-type Homo sapiens
R199C single nucleotide polymorphism, inactive Homo sapiens
S17T single nucleotide polymorphism, enzymic activities similar to wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 benzoyl-CoA mutant K16N, pH 8.0, 37°C Homo sapiens
0.024
-
 benzoyl-CoA wild-type, pH 8.0, 37°C Homo sapiens
0.028
-
 benzoyl-CoA mutant S17T, pH 8.0, 37°C Homo sapiens
0.038
-
 benzoyl-CoA mutant N156S, pH 8.0, 37°C Homo sapiens
0.053
-
 benzoyl-CoA mutant F168L, pH 8.0, 37°C Homo sapiens
0.071
-
 benzoyl-CoA mutant R131H, pH 8.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6IB77
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + glycine
-
 Homo sapiens CoA + N-benzoylglycine
-
 ?