Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus | |
0.8 | - |
taurine | + 0.05 mM deoxycholoyl-CoA | Rattus norvegicus | |
0.8 | - |
aminomethanesulfonate | + 0.05 mM deoxycholoyl-CoA | Rattus norvegicus | |
31 | - |
glycine | + 0.05 mM deoxycholoyl-CoA | Rattus norvegicus | |
175 | - |
beta-Alanine | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choloyl-CoA + taurine | Rattus norvegicus | taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine | CoA + taurocholate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Comment) | Organism |
---|---|
partial, 200fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
1.99 | - |
taurine | Rattus norvegicus |
3.88 | - |
glycine | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, several weeks, stable | Rattus norvegicus |
0° to 20°C, several h, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chenodeoxycholoyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycochenodeoxycholate | - |
? | |
chenodeoxycholoyl-CoA + taurine | - |
Rattus norvegicus | CoA + taurochenodeoxycholate | - |
? | |
choloyl-CoA + beta-alanine | - |
Rattus norvegicus | CoA + beta-alanylcholate | - |
? | |
choloyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | preferred substrate | Rattus norvegicus | CoA + taurocholate | - |
? | |
choloyl-CoA + taurine | taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine | Rattus norvegicus | CoA + taurocholate | - |
? | |
deoxycholoyl-CoA + aminomethanesulfonate | - |
Rattus norvegicus | CoA + aminomethanesulfonyldeoxycholate | - |
? | |
deoxycholoyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycodeoxycholate | - |
? | |
deoxycholoyl-CoA + taurine | - |
Rattus norvegicus | CoA + taurodeoxycholate | - |
? | |
lithocholoyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycolithocholate | - |
? | |
lithocholoyl-CoA + taurine | - |
Rattus norvegicus | CoA + taurolithocholate | - |
? | |
additional information | not: D-alanine, L-arginine, L-lysine, 2-hydroxyethanesulfonate, 2-aminoethanesulfonate, 1-aminoethanephosphonate and 2-aminoethanephosphonate, 3-aminopropanesulfonate | Rattus norvegicus | ? | - |
? | |
additional information | not as acyl-CoA donors: acetyl-CoA, phenylacetyl-CoA and benzoyl-CoA | Rattus norvegicus | ? | - |
? | |
additional information | not as acyl-CoA donors: succinoyl-CoA, palmitoyl-CoA | Rattus norvegicus | ? | - |
? | |
additional information | not: L-alpha-alanine, L-glutamine, L-ornithine | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable below | Rattus norvegicus |
50 | - |
inactivation above | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 7.2 | Rattus norvegicus |
7.8 | 8 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 70% of maximal activity at pH 6.0, about half-maximal activity at pH 9.0, inactivation rate is similar for both taurine and glycine | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
phosphate buffer, t1/2: 2 h | Rattus norvegicus |
9 | - |
Tris-buffer, t1/2: 3 h | Rattus norvegicus |