Literature summary for 2.3.1.61 extracted from

Koike, K.; Suematsu, T.; Ehara, M.
Cloning, overexpression and mutagenesis of cDNA encoding dihydrolipoamide succinyltransferase component of the porcine 2-oxoglutarate dehydrogenase complex (2000), Eur. J. Biochem., 267, 3005-3016.

Search for more literature for 2.3.1.61

Cloned(Commentary)

Cloned (Comment)	Organism
cDNA encoding porcine E20 cloned, with expression vector pET-11d overexpressed in Escherichia coli	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	succinyl-CoA	-	Sus scrofa
4.2	-	dihydrolipoamide	-	Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41530	-	amino acid sequence	Sus scrofa
48000	-	24 * 48000, SDS-PAGE	Sus scrofa
1000000	-	-	Escherichia coli
1000000	-	gel filtration, FPLC	Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
succinyl-CoA + dihydrolipoamide	Bacillus subtilis	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Escherichia coli	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Homo sapiens	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Rattus norvegicus	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Sus scrofa	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Saccharomyces cerevisiae	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Bos taurus	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Enterococcus faecalis	-	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	Azotobacter vinelandii	-	CoA + S-succinyldihydrolipoamide	-	?

Organism

Organism	UniProt	Comment	Textmining
Azotobacter vinelandii	-	-	-
Bacillus subtilis	-	-	-
Bos taurus	-	-	-
Enterococcus faecalis	-	-	-
Escherichia coli	-	-	-
Homo sapiens	-	-	-
Rattus norvegicus	-	-	-
Saccharomyces cerevisiae	-	yeast	-
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Sus scrofa	-
heart	-	Homo sapiens	-
heart	-	Rattus norvegicus	-
heart	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-CoA + dihydrolipoamide	-	Bacillus subtilis	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Escherichia coli	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Homo sapiens	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Rattus norvegicus	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Sus scrofa	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Saccharomyces cerevisiae	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Bos taurus	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Enterococcus faecalis	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + dihydrolipoamide	-	Azotobacter vinelandii	CoA + S-succinyldihydrolipoamide	-	?

Subunits

Subunits	Comment	Organism
polymer	-	Escherichia coli
polymer	24 * 48000, SDS-PAGE	Sus scrofa

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Release 2023.1 (January 2023)