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Literature summary for 2.3.1.54 extracted from

  • Wang, Q.; Ou, M.S.; Kim, Y.; Ingram, L.O.; Shanmugam, K.T.
    Metabolic Flux Control at the Pyruvate Node in an Anaerobic Escherichia coli with Active Pyruvate Dehydrogenase (2010), Appl. Environ. Microbiol., 76, 2107-2114.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.54

Cloned(Commentary)

Cloned (Comment) Organism
gene deletion is transduced via Bacteriophage P1 Escherichia coli str. K-12 substr. W3110

Protein Variants

Protein Variants Comment Organism
additional information mutant strain YK167 lacking pyruvate formate-lyase shows almost 50% increase in glucose consumption and flux with higher flux to lactate and lower ATP-yield, double mutant for pyruvate formate-lyase and lactate dehydrogenase (strain SE2382) shows 50% lower glucose flux and ATP yield than strain YK167 and results in reduced cell mass growth Escherichia coli str. K-12 substr. W3110

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + pyruvate Escherichia coli str. K-12 substr. W3110 anaerobic growth under nitrogen, 37°C, pH 7.0, extract enzyme reactions measured at room temperature acetyl-CoA + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli str. K-12 substr. W3110
-
 wild-type and mutant strains, anaerobic growth conditions
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation, washing in 50 mM potassium phosphate buffer, pH 7.5, resuspension, French press disruption, centrifugation of crude extract and of resulting supernatant Escherichia coli str. K-12 substr. W3110

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + pyruvate anaerobic growth under nitrogen, 37°C, pH 7.0, extract enzyme reactions measured at room temperature Escherichia coli str. K-12 substr. W3110 acetyl-CoA + formate
-
 ?

Synonyms

Synonyms Comment Organism
PFL
-
 Escherichia coli str. K-12 substr. W3110
pyruvate formate-lyase
-
 Escherichia coli str. K-12 substr. W3110

General Information

General Information Comment Organism
metabolism anaerobic fermentation pathway yielding 3 ATP per glucose, pyruvate channeling to fermentation product, lactate dehydrogenase and pyruvate dehydrogenase are alternative pyruvate channeling enzymes during fermentation Escherichia coli str. K-12 substr. W3110