Literature summary for 2.3.1.50 extracted from

Hornemann, T.; Penno, A.; Ruetti, M.F.; Ernst, D.; Kivrak-Pfiffner, F.; Rohrer, L.; von Eckardstein, A.
The SPTLC3 subunit of serine palmitoyltransferase generates short chain sphingoid bases (2009), J. Biol. Chem., 284, 26322-26330.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of SPTLC3 in HEK-293 cells leads to the formation of two new sphingoid base metabolites, namely C16-sphinganine and C16-sphingosine. SPTLC3-expressing cells have higher in vitro SPT activities with lauryl- and myristoyl-CoA than SPTLC2-expressing cells, and SPTLC3 mRNA expression levels correlate closely with the C16-sphinganine synthesis rates in various human and murine cell lines. Quantitative reverse transcription--PCR analysis	Homo sapiens

Cloned (Comment)

Organism

overexpression of SPTLC3 in HEK-293 cells leads to the formation of two new sphingoid base metabolites, namely C16-sphinganine and C16-sphingosine. SPTLC3-expressing cells have higher in vitro SPT activities with lauryl- and myristoyl-CoA than SPTLC2-expressing cells, and SPTLC3 mRNA expression levels correlate closely with the C16-sphinganine synthesis rates in various human and murine cell lines. Quantitative reverse transcription--PCR analysis

Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	kinetics of the recombinant SPTLC3 subunit in HEK-293 cells, overview	Homo sapiens
0.03	-	myristoyl-CoA	pH 8.0, 37°C, recombinant SPTLC3	Homo sapiens
0.04	-	palmitoyl-CoA	pH 8.0, 37°C, recombinant SPTLC3	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
myristoyl-CoA + L-serine	Homo sapiens	recombinant SPTLC3 subunit in HEK-293 cells	CoA + ? + CO2	-	?
palmitoyl-CoA + L-serine	Homo sapiens	-	CoA + 3-dehydro-D-sphinganine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	SPT contains subunits SPTLC1-SPTLC3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
myristoyl-CoA + L-serine	-	Homo sapiens	CoA + ? + CO2	-	?
myristoyl-CoA + L-serine	recombinant SPTLC3 subunit in HEK-293 cells	Homo sapiens	CoA + ? + CO2	-	?
palmitoyl-CoA + L-serine	-	Homo sapiens	CoA + 3-dehydro-D-sphinganine + CO2	-	?

Synonyms

Synonyms	Comment	Organism
serine palmitoyltransferase	-	Homo sapiens
SPT	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	all subunits contain a pyridoxal 5'-phosphate consensus motif sequence	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	SPT catalyzes the rate-limiting step in the de novo synthesis of sphingolipids, subunit SPTLC3 generates C16-sphingoid bases, and sphingolipids with a C16 backbone constitute a significant proportion of human plasma sphingolipids	Homo sapiens