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Literature summary for 2.3.1.50 extracted from
- Human and murine serine-palmitoyl-CoA transferase. Cloning, expression and characterization of the key enzyme in sphingolipid synthesis (1997), Eur. J. Biochem., 249, 239-247.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| expression of mLCB1 and LCB2 as glutathione-S-transferase fusion protein in Escherichia coli DH5alpha | Mus musculus |
| hLCB1 and hLCB2 | Homo sapiens |
| in vitro transcription and translation of mLCB2 | Mus musculus |
| overexpression of mLCB1 and mLCB2 in human embryonic kidney cells, independently and in coexpression with mLCB1 | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-chloro-L-alanine | - |
Homo sapiens |  |
| beta-chloro-L-alanine | - |
Mus musculus | |
| L-Cycloserine | - |
Homo sapiens | |
| L-Cycloserine | - |
Mus musculus | |
| lipoxamycin | - |
Homo sapiens | |
| lipoxamycin | - |
Mus musculus | |
| myriocin | - |
Homo sapiens | |
| myriocin | - |
Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-serine | Mus musculus | first step of biosynthesis of sphingolipid bases | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | Homo sapiens | first step of biosynthesis of sphingolipid bases | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | - |
gene hLCB1 and hLCB2 | - |
| Mus musculus | - |
gene mLCB2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Mus musculus | - |
| kidney | human embryonic kidney cell line HEK 293 | Homo sapiens | - |
| lung | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000018 | - |
HEK cell extract | Homo sapiens |
| 0.000044 | - |
HEK cells transfected with mLCB2, crude extract | Mus musculus |
| 0.000044 | - |
HEK cells transfected with mLCB2, crude extract | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-serine | - |
Mus musculus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | - |
Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | first step of biosynthesis of sphingolipid bases | Mus musculus | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| palmitoyl-CoA + L-serine | first step of biosynthesis of sphingolipid bases | Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
| pyridoxal 5'-phosphate | - |
Homo sapiens | |
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