Literature summary for 2.3.1.5 extracted from

Xu, X.; Li de la Sierra-Gallay, I.; Kubiak, X.; Duval, R.; Chaffotte, A.F.; Dupret, J.M.; Haouz, A.; Rodrigues-Lima, F.
Insight into cofactor recognition in arylamine N-acetyltransferase enzymes: structure of Mesorhizobium loti arylamine N-acetyltransferase in complex with coenzyme A (2015), Acta Crystallogr. Sect. D, 71, 266-273.

Search for more literature for 2.3.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mesorhizobium loti

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with CoA, sitting drop vapor diffusion method, using 20% (w/v) PEG 4000, 0.6 M NaCl, 0.1 M MES buffer pH 6.5	Mesorhizobium loti

Protein Variants

Protein Variants	Comment	Organism
F42W	the mutant displays enzymatic properties similar to those of the wild type enzyme	Mesorhizobium loti

Organism

Organism	UniProt	Comment	Textmining
Mesorhizobium loti	Q98D42	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Mesorhizobium loti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + 4-aminosalicylate	-	Mesorhizobium loti	CoA + N-acetyl-4-aminosalicylate	-	?

Synonyms

Synonyms	Comment	Organism
NAT1	-	Mesorhizobium loti

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
9	-	4-Aminosalicylate	at pH 7.5 and 25°C	Mesorhizobium loti
15	-	acetyl-CoA	at pH 7.5 and 25°C	Mesorhizobium loti

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Release 2023.1 (January 2023)