Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant NAT2 in Escherichia coli, the mutant variants are insoluble, inactive enzymes | Mesocricetus auratus |
Protein Variants | Comment | Organism |
---|---|---|
H107N | site-directed mutagenesis, no expression in Escherichia coli cells possible | Mesocricetus auratus |
H107Q | site-directed mutagenesis, no expression in Escherichia coli cells possible, insoluble inactive enzyme | Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, kinetic and isotope effect studies | Mesocricetus auratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an arylamine | Mesocricetus auratus | - |
CoA + an N-acetylarylamine | - |
? | |
additional information | Mesocricetus auratus | the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | P50293 | isozyme NAT2; isozyme NAT2 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant NAT2 from Escherichia coli by anion exchange chromatography | Mesocricetus auratus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine | ping pong reaction mechanism, the enzyme contains a strictly conserved a Cys-His-Asp catalytic triad, acetylation of the hamster NAT2 is dependent on a thiolate-imidazolium ion pair, Cys-S--His-ImH+, and not a general acid-base catalysis, a deacetylation mechanism dominated by nucleophilic attack of the thiol ester for arylamines | Mesocricetus auratus |
Renatured (Comment) | Organism |
---|---|
recovery of 25-50% activity of the recombinant wild-type enzyme after expression in Escherichia coli | Mesocricetus auratus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + 4-aminobenzoic acid | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + 4-nitroaniline | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-4-nitroaniline | - |
? | |
4-nitrophenyl acetate + anisidine | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-anisidine | - |
? | |
4-nitrophenyl acetate + N-(4-aminobenzoyl)-L-glutamate | - |
Mesocricetus auratus | 4-nitrophenol + N-(4-acetylaminobenzoyl)-L-glutamate | - |
? | |
acetyl-CoA + 4-aminobenzoic acid | preferred substrate | Mesocricetus auratus | CoA + N-acetyl-4-aminobenzoic acid | - |
? | |
acetyl-CoA + 4-aminobenzoyl-L-glutamic acid | - |
Mesocricetus auratus | CoA + 4-acetylaminobenzoyl-L-glutamic acid | - |
? | |
acetyl-CoA + 4-nitroaniline | - |
Mesocricetus auratus | CoA + N-acetyl-4-nitroaniline | - |
? | |
acetyl-CoA + an arylamine | - |
Mesocricetus auratus | CoA + an N-acetylarylamine | - |
? | |
acetyl-CoA + anisidine | - |
Mesocricetus auratus | CoA + N-acetyl-anisidine | - |
? | |
additional information | the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics | Mesocricetus auratus | ? | - |
? | |
additional information | 4-aminobenzoic acid is the preferred substrate | Mesocricetus auratus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arylamine N-acetyltransferase 2 | - |
Mesocricetus auratus |
NAT | - |
Mesocricetus auratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mesocricetus auratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
4-nitroaniline | pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme | Mesocricetus auratus | |
76 | - |
4-aminobenzoyl glutamic acid | pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme | Mesocricetus auratus | |
120 | - |
4-aminobenzoyl glutamic acid | pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme | Mesocricetus auratus | |
200 | - |
4-Aminobenzoic acid | pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme | Mesocricetus auratus | |
260 | - |
anisidine | pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme | Mesocricetus auratus | |
620 | - |
4-Aminobenzoic acid | pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mesocricetus auratus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 9 | pH profiles with cofactors acetyl-CoA and 4-nitrophenyl acetate, overview | Mesocricetus auratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mesocricetus auratus |