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Literature summary for 2.3.1.48 extracted from

  • Wang, J.; Dixon, S.E.; Ting, L.M.; Liu, T.K.; Jeffers, V.; Croken, M.M.; Calloway, M.; Cannella, D.; Hakimi, M.A.; Kim, K.; Sullivan, W.J.
    Lysine acetyltransferase GCN5b interacts with AP2 factors and is required for Toxoplasma gondii proliferation (2014), PLoS Pathog., 10, e1003830.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the inducible HA-tagged wild-type enzyme and dominant-negative mutant E703G in Toxoplasma gondii parasites Toxoplasma gondii

Protein Variants

Protein Variants Comment Organism
E703G the point mutation ablates enzymatic activity, dominant-negative form of GCN5b. Replication arrest observed in ddHAGCN5b(E703G) parasites is due to a reduction in histone acetylation, which in turn leads to dysregulation of the transcriptome Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + histone H3 Toxoplasma gondii
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CoA + acetylhistone H3
-
?
additional information Toxoplasma gondii enzyme GCN5b interacts with AP2-domain proteins, apicomplexan plant-like transcription factors, as well as a core complex that includes the co-activator ADA2-A, TFIID subunits, LEO1 polymerase-associated factor (Paf1) subunit, and RRM proteins ?
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?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii S8F3V2
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-

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
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Toxoplasma gondii
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone H3
-
Toxoplasma gondii CoA + acetylhistone H3
-
?
additional information enzyme GCN5b interacts with AP2-domain proteins, apicomplexan plant-like transcription factors, as well as a core complex that includes the co-activator ADA2-A, TFIID subunits, LEO1 polymerase-associated factor (Paf1) subunit, and RRM proteins Toxoplasma gondii ?
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?

Synonyms

Synonyms Comment Organism
GCN5b
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Toxoplasma gondii

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
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Toxoplasma gondii

General Information

General Information Comment Organism
evolution the enzyme belongs to the GCN5-family of lysine acetyltransferases Toxoplasma gondii
malfunction induced accumulation of the ddHAGCN5b(E703G) protein leads to a rapid arrest in parasite replication. Growth arrest is accompanied by a decrease in histone H3 acetylation at specific lysine residues as well as reduced expression of GCN5b target genes in GCN5b(E703G) parasites, which are identified using chromatin immunoprecipitation coupled with microarray hybridization Toxoplasma gondii
additional information mechanism by which GCN5b is recruited to target genes by co-purifying the enzyme with plant-like AP2-domain proteins, a subset of which function as DNA-binding transcription factors in Apicomplexa, overview Toxoplasma gondii
physiological function enzyme GCN5b seems to be essential for Toxoplasma viability. GCN5b plays a central role in transcriptional and chromatin remodeling complexes. GCN5b has a non-redundant and indispensable role in regulating gene expression required during the Toxoplasma lytic cycle GCN5b is an essential driver of tachyzoite proliferation Toxoplasma gondii