Any feedback?
Literature summary for 2.3.1.48 extracted from
- Modulating substrate specificity of histone acetyltransferase with unnatural amino acids (2011), Mol. Biosyst., 7, 3050-3055.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | residue-specific incorporation of ortho-, meta- and para-fluorophenylalanine results in different effects on the selectivity of human histone acetyltransferase protein, PCAF. Varying the position of the fluorine group in the phenylalanine ring confers different effects on the ability of PCAF to acetylate target histone H3 as well as non-histone p53. Incorporation of p-fluorophenylalanine leads to an increase in activity for non-histone p53, while incorporation of m-fluorophenylalanine results in selectivity for histone H3 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
histone H3 | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.43 | - |
histone H3 | mutant having m-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.7 | - |
histone H3 | mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 1.28 | - |
protein p53 | mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 4.63 | - |
protein p53 | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q09472 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + histone H3 | - |
Homo sapiens | CoA + acetylhistone H3 | - |
? |  |
| acetyl-CoA + protein p53 | - |
Homo sapiens | CoA + acetylprotein p53 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| p300/CBP associated factor | - |
Homo sapiens |
| PCAF | - |
Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
protein p53 | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 0.068 | - |
protein p53 | mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 2.73 | - |
histone H3 | mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 7.48 | - |
histone H3 | mutant having m-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication | Homo sapiens | |
| 9.73 | - |
histone H3 | wild-type, pH 8.0, temperature not specified in the publication | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
