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Literature summary for 2.3.1.48 extracted from

  • Decker, P.V.; Yu, D.Y.; Iizuka, M.; Qiu, Q.; Smith, M.M.
    Catalytic-site mutations in the MYST family histone acetyltransferase Esa1 (2008), Genetics, 178, 1209-1220.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged wild-type and mutant Esa1s in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C304S site-directed mutagenesis, the mutant shows 97.4% reduced histone H4 acetylation at pH 8.0 compared to the wild-type Esa1 Saccharomyces cerevisiae
C304S/E338Q site-directed mutagenesis, the mutant protein is intrinsically unstable and catalytically inactive, the mutant cell is lethal Saccharomyces cerevisiae
D343V site-directed mutagenesis, the mutant shows reduced histone H4 acetylation compared to the wild-type Esa1 Saccharomyces cerevisiae
E338Q site-directed mutagenesis, the mutant shows 99.5% reduced histone H4 acetylation at pH 8.0 compared to the wild-type Esa1, recombinant Esa1-E338Q displays detectable HATactivity at elevated pH of 9.2 Saccharomyces cerevisiae
L357H site-directed mutagenesis, the mutant shows 41% reduced histone H4 acetylation compared to the wild-type Esa1 Saccharomyces cerevisiae
W66R site-directed mutagenesis, the mutant shows reduced histone H4 acetylation compared to the wild-type Esa1 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae Esa1 is the catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo NuA4, picNuA4 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 diverse strains
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone H3
-
 Saccharomyces cerevisiae CoA + acetylhistone H3
-
 ?
additional information Esa1 is the catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo NuA4, picNuA4 Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Esa1
-
 Saccharomyces cerevisiae
HAT
-
 Saccharomyces cerevisiae
More the enzyme belongs to the MYST acetyltransferase family Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.2 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function Esa1 catalytic HAT activity is essential in yeast binding acetyl-CoA or lysine substrates and positively regulating the activities of NuA4 and Piccolo NuA4, Esa1 is involved in DNA damage repair Saccharomyces cerevisiae