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Literature summary for 2.3.1.48 extracted from

  • Langer, M.R.; Fry, C.J.; Peterson, C.L.; Denu, J.M.
    Modulating acetyl-CoA binding in the GCN5 family of histone acetyltransferases (2002), J. Biol. Chem., 277, 27337-27344.
    View publication on PubMed
Search for more literature for 2.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
expression of Gcn5 and mutants thereof in Escherichia coli BL21 (DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A190S site-directed mutagenesis, mutant of Gcn5, similar kinetics to wild-type Saccharomyces cerevisiae
A190T site-directed mutagenesis, mutant of Gcn5, decreased Km for acetyl-CoA Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00058
-
 acetyl-CoA Gcn5 Tetrahymena thermophila
0.00062
-
 acetyl-CoA Gcn5 Homo sapiens
0.0007
-
 acetyl-CoA Gcn5 mutant A190T Saccharomyces cerevisiae
0.00098
-
 acetyl-CoA PCAF protein Homo sapiens
0.0021
-
 acetyl-CoA Gcn5 mutant A190S Saccharomyces cerevisiae
0.0025
-
 acetyl-CoA Gcn5 protein Saccharomyces cerevisiae
0.273
-
 histone H3 Gcn5 mutant A190S Saccharomyces cerevisiae
0.352
-
 histone H3 Gcn5 mutant A190T Saccharomyces cerevisiae
0.357
-
 histone H3 Gcn5 Tetrahymena thermophila
0.471
-
 histone H3 Gcn5 Homo sapiens
0.49
-
 histone H3 Gcn5 Saccharomyces cerevisiae
0.532
-
 histone H3 PCAF Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence comparison, Gcn5 Homo sapiens
additional information
-
 amino acid sequence comparison, Gcn5 Saccharomyces cerevisiae
additional information
-
 amino acid sequence comparison, Gcn5 Tetrahymena thermophila

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 GCN5
-
Homo sapiens
-
 PCAF protein
-
Homo sapiens GCN5
-
 GCN5
-
Saccharomyces cerevisiae
-
 gene Gcn5
-
Tetrahymena thermophila
-
 GCN5
-
Tetrahymena thermophila GCN5
-
 GCN5
-

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine mechanisms Homo sapiens
a
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine mechanisms Saccharomyces cerevisiae
a
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine mechanisms Tetrahymena thermophila
a
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine binding motifs Homo sapiens
a
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine binding motifs Saccharomyces cerevisiae
a
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine binding motifs Tetrahymena thermophila
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone
-
 Homo sapiens CoA + acetylhistone
-
 ?
acetyl-CoA + histone
-
 Saccharomyces cerevisiae CoA + acetylhistone
-
 ?
acetyl-CoA + histone
-
 Tetrahymena thermophila CoA + acetylhistone
-
 ?
acetyl-CoA + histone
-
 Homo sapiens GCN5 CoA + acetylhistone
-
 ?
acetyl-CoA + histone
-
 Tetrahymena thermophila GCN5 CoA + acetylhistone
-
 ?
acetyl-CoA + histone H3-peptide
-
 Homo sapiens CoA + acetylhistone H3 -peptide
-
 ?
acetyl-CoA + histone H3-peptide
-
 Saccharomyces cerevisiae CoA + acetylhistone H3 -peptide
-
 ?
acetyl-CoA + histone H3-peptide
-
 Homo sapiens GCN5 CoA + acetylhistone H3 -peptide
-
 ?

Synonyms

Synonyms Comment Organism
HAT
-
 Homo sapiens
HAT
-
 Saccharomyces cerevisiae
HAT
-
 Tetrahymena thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0267
-
 acetyl-CoA mutant A190S and A190T of Gcn5 Saccharomyces cerevisiae
0.0267
-
 histone H3 mutant A190S and A190T of Gcn5 Saccharomyces cerevisiae
0.0267
-
 acetyl-CoA Gcn5 Tetrahymena thermophila
0.0267
-
 histone H3 Gcn5 Tetrahymena thermophila
0.0283
-
 acetyl-CoA Gcn5 protein Homo sapiens
0.0283
-
 acetyl-CoA Gcn5 protein Saccharomyces cerevisiae
0.0283
-
 histone H3-peptide Gcn5 protein Homo sapiens
0.0283
-
 histone H3-peptide Gcn5 protein Saccharomyces cerevisiae
0.0383
-
 acetyl-CoA PCAF Homo sapiens
0.0383
-
 histone H3-peptide PCAF Homo sapiens