Literature summary for 2.3.1.47 extracted from

Alexeev, D.; Alexeeva, M.; Baxter, R.L.; Campopiano, D.J.; Webster, S.P.; Sawyer, L.
The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme (1998), J. Mol. Biol., 284, 401-419.

Search for more literature for 2.3.1.47

Cloned(Commentary)

Cloned (Comment)	Organism
bioF gene overexpression in Escherichia coli	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
pH 8.5-8.9, ammonium sulfate precipitation, apoenzyme, X-ray diffraction structure analysis with and without bound pyridoxyl-5'-phosphate, model, active site residues	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	amino acid sequence	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-carboxyhexanoyl-CoA + L-alanine	Escherichia coli	i.e. pimeloyl-CoA	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	?
6-carboxyhexanoyl-CoA + L-alanine	Escherichia coli	reaction in biotin biosynthesis	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P12998	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from E. coli	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]	reaction mechanism	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-carboxyhexanoyl-CoA + L-alanine	i.e. pimeloyl-CoA	Escherichia coli	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	?
6-carboxyhexanoyl-CoA + L-alanine	reaction in biotin biosynthesis	Escherichia coli	8-amino-7-oxononanoate + CoA + CO2	i.e. 7-oxo-8-amino-pelargonic acid	?

Subunits

Subunits	Comment	Organism
More	dimer, active site is formed at the interface between the monomers	Escherichia coli

Synonyms

Synonyms	Comment	Organism
AONS	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Escherichia coli

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Release 2023.1 (January 2023)