Cloned (Comment) | Organism |
---|---|
expressed in overproducing Escherichia coli strain C268/pTG3401 | Lysinibacillus sphaericus |
General Stability | Organism |
---|---|
EDTA stabilizes during purification | Lysinibacillus sphaericus |
freeze-thawing inactivates | Lysinibacillus sphaericus |
proteinase inhibitors, e.g. PMSF, pepstatin A, stabilize during purification | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
pimeloyl-CoA | - |
Lysinibacillus sphaericus | |
3 | - |
L-alanine | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Lysinibacillus sphaericus |
41000 | - |
1 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
46000 | - |
gel filtration | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | i.e. pimeloyl-CoA | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | reaction in biotin biosynthesis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
gene bioF | - |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli overproducing strain | Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.67 | - |
- |
Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
0°C, several months | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | i.e. pimeloyl-CoA | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | reaction in biotin biosynthesis | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
AOP synthase | - |
Lysinibacillus sphaericus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lysinibacillus sphaericus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
pimeloyl-CoA | - |
Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.2 | Lysinibacillus sphaericus |
7 | - |
assay at | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Lysinibacillus sphaericus |