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Literature summary for 2.3.1.46 extracted from

  • Mordukhova, E.A.; Pan, J.G.
    Stabilization of homoserine-O-succinyltransferase (MetA) decreases the frequency of persisters in Escherichia coli under stressful conditions (2014), PLoS ONE, 9, e110504.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.46

Protein Variants

Protein Variants Comment Organism
additional information the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth. Overexpression of MetA induces the greatest number of persisters at 42°C, which is correlated to an increased level of aggregated MetA. Substitution of the native metA gene on the Escherichia coli K-12 WE chromosome by a mutant gene encoding the stabilized MetA leads to reduction in persisters at the elevated temperature and in the presence of acetate, as well as lower aggregation of the mutated MetA. Decreased persister formation at 42°C is confirmed also in wild-type Escherichia coli K-12 W3110 and a fast-growing WErph+ mutant harboring the stabilized MetA. The frequency of persister formation is correlated to the aggregation of the MetA, more than 150fold increase in the frequency of persisters at 42°C result from an increased level of aggregated proteins. Stabilized MetA reduces persister formation in the presence of acetate Escherichia coli

General Stability

General Stability Organism
inherently unstable and prone to aggregation, the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + L-homoserine Escherichia coli
-
 CoA + O-succinyl-L-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P07623 gene metA
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + L-homoserine
-
 Escherichia coli CoA + O-succinyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
homoserine-O-succinyltransferase
-
 Escherichia coli
MetA
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
 complete aggregation of wild-tyype enzyyme at 44°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
succinyl-CoA
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism homoserine-o-succinyltransferase catalyzes the first unique step in the de novo methionine biosynthetic pathway Escherichia coli
additional information the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth Escherichia coli