Any feedback?
Literature summary for 2.3.1.41 extracted from
- Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB (2009), J. Biol. Chem., 284, 29526-29535.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | introduction of temperature-sensitive mutation in fabB into strain K27 by phage P1-mediated transduction using closely linked Tn10 insertion as selectable marker. A fabB mutant strain accumulates less cis-5-dodecenoic acid than the parental wild-type strain. The wild-type strain also accumulates 2.7fold more cis-7-tetradecenoate than the fabB(Ts) strain. The combination of null mutations in fadD and fabA or fabB is synthetically lethal even in the presence of oleic acid supplementation. A temperature-sensitive mutation is used rather than null mutations because a strain having fabB null mutation has an obligate requirement for an unsaturated fatty acid and FabD is required for uptake of the supplement | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein | 3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein | the product is not incorporated into the complex lipids of the bacterium | ? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene fabB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein | - |
? | |
| cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein | Escherichia coli | 3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein | the product is not incorporated into the complex lipids of the bacterium | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-ketoacyl-ACP synthase | - |
Escherichia coli |
| FabB | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | FabB catalyzes the elongation of cis-3-decenoyl-ACP produced by FabA to generate the first 12-carbon cis-unsaturated fatty acid intermediate, 3-oxo-cis-5-decenoyl-ACP, which is converted to cis-5-dodecenoyl-ACP. FabB is essential in the unsaturated fatty acid, UFA, synthesis pathway, strains lacking FabB are UFA auxotroph, overview. FabB cannot be substituted by FabF | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
