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Literature summary for 2.3.1.41 extracted from
- beta-ketoacyl-acyl carrier protein synthase III (FabH) is a determining factor in branched-chain fatty acid biosynthesis (2000), J. Bacteriol., 182, 365-370.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged FabH1 and FabH2 in Escherichia coli INValphaF' | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence alignment | Bacillus subtilis |
| additional information | - |
amino acid sequence alignment | Escherichia coli |
| 39800 | - |
x * 39800, FabH2, SDS-PAGE | Bacillus subtilis |
| 42700 | - |
x * 42700, FabH1, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | - |
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |  |
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Bacillus subtilis | initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
genes fabH1 and fabH2, 2 synthase III isozymes | - |
| Escherichia coli | - |
synthase III, gene fabH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged proteins FabH1 and FabH2 from E. coli | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus subtilis |
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity | Bacillus subtilis | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| additional information | - |
Escherichia coli | ? | - |
? | |
| additional information | synthase III isozymes also utilize iso- and anteiso-branched-chain acyl-CoA primers as substrates | Bacillus subtilis | ? | - |
? | |
| saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Bacillus subtilis | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42700, FabH1, SDS-PAGE | Bacillus subtilis |
| ? | x * 39800, FabH2, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bacillus subtilis |
| 7 | - |
assay at | Escherichia coli |
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