Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.41 extracted from

  • Toomey, R.E.; Wakil, S.J.
    Studies on the mechanism of fatty acid synthesis. XVI. Preparation and general properties of acyl-malonyl acyl carrier protein-condensing enzyme from Escherichia coli (1966), J. Biol. Chem., 241, 1159-1165.
    View publication on PubMed
Search for more literature for 2.3.1.41

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Escherichia coli

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Escherichia coli
EDTA stabilizes Escherichia coli
unstable during purification Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA
-
 Escherichia coli
acyl-CoA at high concentrations Escherichia coli
iodoacetamide acyl-[acyl-carrier-protein]-derivatives, e.g. acetyl-[acyl-carrier-protein], hexanoyl-CoA, octanoyl-CoA, decanoyl-CoA protect Escherichia coli
N-ethylmaleimide acyl-[acyl-carrier-protein]-derivatives protect Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67600
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Escherichia coli part of non-associated fatty acid synthase system of plants and prokaryotes 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] Escherichia coli involved in biosynthesis of saturated fatty acids 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.403
-
 purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, complete loss of activity in the presence of EDTA and 2-mercaptoethanol Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] part of non-associated fatty acid synthase system of plants and prokaryotes Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] involved in biosynthesis of saturated fatty acids Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli