Cloned (Comment) | Organism |
---|---|
gene fabD, expression of His-tagged enzyme in Escherichia coli strain BL21 | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
recombinant purified enzyme, 3 mg/ml protein in 10 mM Tris-HCl, pH 7.4, 1 mM 2-mercaptoethanol, hanging drop vapoir diffusion method, 3 days, room temperature, soaking of crystals in 30% PEG 4000, 100 mM sodium acetate, pH 4.8, 200 mM ammonium acetate, and 20% glycerol for cryoprotection, X-ray diffraction structure determination and analysis at 2.0 A resolution, macromolecular docking simulation with K47A/K190A/R287A/K293A mutant actinorhodin ACP structure, overview | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | Streptomyces coelicolor | enzyme is involved in both fatty acid and polyketide synthesis pathways | CoA + malonyl-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
gene fabD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity | Streptomyces coelicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] | catalytic mechanism, [acyl-carrier protein] binding site, residues Phe200 and Met126 are involved | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | - |
Streptomyces coelicolor | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + [acyl-carrier protein] | enzyme is involved in both fatty acid and polyketide synthesis pathways | Streptomyces coelicolor | CoA + malonyl-[acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA:ACP transacylase | - |
Streptomyces coelicolor |
MAT | - |
Streptomyces coelicolor |